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Basic information of glucosidase

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Basic information of glucosidase

Desheng company is established for 15 years, focusing on R & D, production and sales in one of the old company, R & D and production of a complete range of products, price concessions, now on our company's R & D and production experience α- Glucosidase is a simple explanation.

 

Glucosidase is one of the major enzymes in glycoside hydrolases (EC 3.2.1). It is named because it can hydrolyze glucoside bond and release a molecule of glucose.

 

Glucosidase is one of the important members of glucose metabolism pathway. β- Glucosidase is involved in the metabolism of cellulose and many physiological and biochemical pathways, α- Glucosidase is directly involved in the metabolism of starch and glycogen. It has double functions of hydrolysis and transglycoside in the catalytic reaction of sugar. Hydrolysis can be carried out from α- Non reducing telostomy of glucosides, oligosaccharides and glucans α- 1,4 glycosidic bond, releasing glucose; In addition, the free glucose residues can be separated by transglycosylation α- Non fermentative Isomaltooligosaccharides (IMO) were obtained by transferring 1,6 glycosidic bond to another glucose or maltose substrate. The abnormal function of these enzymes will lead to metabolic diseases. At the same time, these enzymes are also the targets of many drugs and inhibitors to regulate the glycochemical metabolism in the human body.

 

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α- Glucosidase

α- Glucosidase can be used to screen active natural drugs;

one α- Immobilization of glucosidase: using trimethylol phosphorus as crosslinking agent and chitosan as carrier α- Glucosidase;

 

2. Make inhibitor screening model: immobilize the above-mentioned inhibitors α- The glucosidase was loaded into a column with a diameter of 0.8 cm and a length of 8 cm, in which the pH 6.8 potassium phosphate buffer was added and stored at 4 ℃;

 

3. Validation of the model: a representative α- Acarbose, a glucosidase inhibitor, was used to verify the effectiveness of the screening model. The lower end of the column was tightly inserted into a small tube with a piston, and a syringe was inserted into the upper end. The buffer solution in the column was pressed to about 0.4cm below the top of the column, and then 50 μ L 4-nitrobenzene- α- D-glucopyranoside (0.116mol / L) and a certain amount of acarbose solution (250mg / L), gently shake to make the solution at the top of the column mix well, then continue to press the solution until the liquid level is level with the top of the column, close the bottom piston, then put the column into the water solution tank, incubate at 37 ℃ for 10 minutes, after the reaction is completed, use 5ml Wash the column with ph6.8 potassium phosphate buffer, collect the washing solution, dilute the eluent to 10ml volume, and determine the absorbance value at 400nm with ultraviolet spectrophotometer; And the above 50 μ L 4-nitrobenzene- α- D-glucopyranoside, acarbose solution and 5ml buffer solution were diluted to 10ml as blank, and the inhibition rate of acarbose was calculated according to the absorbance data of the obtained reaction solution;

 

4. Immobilization α- Screening model of glucosidase for water soluble fraction of traditional Chinese medicine Polygonum cuspidatum α- Objective to screen the water-soluble fraction of Polygonum cuspidatum.

We Desheng R & D and production of enzyme preparations used in the kit, customers with relevant needs are welcome to consult.

Pub Time : 2021-05-14 20:15:58 >> News list
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