HEPES is a kind of amphoteric buffer, which belongs to good's buffer. It is an important biochemical agent in biochemical industry and one of the important buffers in the field of biological research. HEPES chemical name: N-hydroxyethyl piperazine-1-ethanesulfonic acid, white crystalline powder, is a weak acid, its pH buffer range is 6.8-8.2, soluble in water, does not form stable complexes with metal ions, in most cases, will not interfere with the biochemical process. Because of its advantages, we can find its trace in many fields.
HEPES (7365-45-9) is a cosmetic ingredient with EWG green certification, which is safe and mild. HEPES has the advantages of safety to human body, non toxicity, good physiological compatibility and stable chemical properties.
HEPES can stabilize the pH range of skin care products for a long time, better maintain the activity of microbial fermentation products extract, and make the anti-aging effect of products last for a long time, so it is often used as a stabilizer and activator in cosmetics. HEPES is also a common penetration enhancer, which can promote the transdermal absorption of various functional components in cosmetics. Compared with azone penetration enhancers, HEPES has the characteristics of short onset time, less dosage and high penetration rate. In addition, in some famous cosmetics such as L'Oreal and Lancome, HEPES is mainly used as a peeling agent to soften cutin and promote the metabolism of skin cells.
HEPES is more commonly used in cell culture, in vitro fertilization and embryo culture buffer, but HEPES can be combined with metal ions, most of the metal ion research is carried out by using HEPES buffer, although Tris and phosphate can also be used for metal ion research, but based on the characteristics of these buffers, it is necessary to choose different directions There will be some differences.
The application range of Desheng HEPES buffer is just in the range of ph6.8-ph8.2, which is in line with the characteristics of the pH value needed for cell culture. Compared with other buffers, HEPES has higher stability in the environment of maintaining the pH value of cell culture medium, so HEPES has higher stability Buffer is widely used in cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell division, living cell imaging and other biological and biochemical research.
Gold nanoparticles were prepared by HEPES NaOH reduction method based on the reducibility of HEPES to excessive metals at a specific pH value. The method is simple, fast, easy to control, less by-products and environmentally friendly.
In protein research, HEPES is often used as the component and eluent of binding buffer in cation exchange chromatography; in DNA research, HEPES is used as the buffer of calcium phosphate and DNA precipitate formation system, AFM and electroporation experiment. In addition, HEPES interferes with the reaction between DNA and restriction enzyme and is not suitable for Lowry's method. HEPES buffer is also used in chemical diagnostic kits, DNA / RNA extraction kits and PCR diagnostic kits.
There are many uses of HEPES waiting for us to discover. Anything of value is worth studying and discovering. Desheng, as a manufacturer of biological buffers, besides HEPES, has a series of buffers such as Tris, Tris HCl, bicine, caps, mops, taps, etc.
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