Taps name n-tri (hydroxymethyl) methyl-3-aminopropane sulfonic acid, CAS No. 29915-38-6, molecular formula c7h17no6s, from the appearance, belongs to white crystal, its content is very high, more than 99%. Trimethylolmethylamino propanesulfonic acid (TAPs) is also a kind of biological buffer. It has good water solubility. The concentration is usually 25g / 50ml. The solution is colorless and transparent, and very clear. The pH value is between 7.7 and 9.1, and the pKa value is 8.4.
Taps is a kind of zwitterionic buffer widely used in biochemistry and molecular biology. It can be used not only as a buffer system in DNA screening system, but also as a buffer component of RNA sample; It can also be used as an important pH stabilizing reagent, which is usually mixed with weak acid and its conjugated base to obtain appropriate pH value.
He is used for electron transfer and phosphorylation of chloroplast thin layer preparation; During the freeze-drying process, oxyhemoglobin can be protected from oxidation to methemoglobin; It can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.
In biochemical experiment projects, taps reacts with most organisms under neutral conditions. The pH value should be between 6 and 8, and the effective buffer range of buffer should also be between 6 and 8. Moreover, it is necessary to ensure that the pH form of buffer reagent cannot chelate with some metal chemical ions. Taps, a buffer used in biochemical detection and molecular diagnosis, has high requirements for reagent purity and impurity ion content.
In the field of clinical diagnosis, taps is also commonly used as a biological buffer. It is usually used in some biochemical diagnostic kits, PCR diagnostic kits and DNA / RNA extraction kits.
Desheng is skilled in technology. Taps buffer is made of high purity and high quality taps. After strict filtration, sterilization and quality testing, it can be directly added to the culture medium. It should be noted that taps buffer, buffer range is ph7.7-9.1, PKA (ion concentration set) is 8.4.
Taps buffer is directly added to the sterile medium or added to the instrument to filter and sterilize the medium. Desheng technology has done a lot of research and improvement in this aspect, and many kinds of biological buffers produced by the company have been recognized by a large number of biochemical testing enterprises and pharmaceutical equipment enterprises.
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