Bicine, N, n-di (hydroxyethyl) glycine, CAS 150-25-4, is a white crystalline powder. Soluble in water, insoluble in acetone, DMAC, DMSO, DMF and other organic solvents.
Bicine is a pH buffer suitable for biochemical and medical research. In the range of pH 7.6 ~ 9.0, the pKa value is 8.3 at 25 ℃. This property makes bicine often used as a buffer solution for capillary electrophoresis. It is also the recommended buffer for low temperature biochemical work. Its molecular structure is similar to glycine. Buffer is recommended in low temperature biochemical work of biological buffer. It was used to prepare stable substrate solution for the determination of serum guaninase. It is also a widely used buffer for enzyme reaction buffer and electrophoresis buffer. The working concentration is 3-100ml
1. Preparation of storage solution for cell culture related experiments: dissolve according to the solubility in the table. If it is used for cell experiment, please prepare the liquid and filter it with 0.22um before adding cells.
2. Preservation of storage liquid: it is recommended to prepare and use the liquid now, and the liquid is not very stable; it can also be packed into a single dosage, which is stable for 2 years. Avoid repeated freezing and thawing.
Since 2005, Desheng technology has been specialized in R & D and production of separation gel, heparin, EDTA potassium salt and other blood collection additives, as well as in vitro diagnostic reagents. It has made deep research on new Trinder's reagents such as chromogenic substrates Mao and toos, biological buffers such as Tris and bicine, and chemiluminescence reagents such as luminol and acridine ester. Our company has a professional independent R & D team and has great potential in synthesis Professional advantages, product quality and technology, at home and abroad has been widely recognized and used by customers. The purity of bicine produced by Desheng technology is more than 99%, with good water solubility and stable process, which can ensure the appearance of pure white crystal powder.
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