The commonly used biological buffers on the market: Tris, Tris HCl, bicine, caps, mops, taps, EPPs, HEPES, PEP, pipes, MOPSO, etc. why do we choose bicine? First, let's look at what bicine is? What are its special advantages?
Bicine is a white crystalline powder with the chemical name of N, N-bis (2-hydroxyethyl) glycine (CAS: 150-25-4) and English name of N, N-bis (2-hydroxyethyl) glycine.
Bicine white crystalline powder
It is soluble in water and insoluble in acetone, DMF, DMSO, DMAC and other organic solvents. N. N-dihydroxyethyl glycine is a pH buffer suitable for biochemical and medical research. The pH buffer range is 7.6-9.0, and the pKa value is 8.3 at 25 ℃. This property makes it often used as a buffer solution for capillary electrophoresis. Its molecular structure is similar to glycine.
It is a widely used buffer. It is not only used to prepare stable substrate solution for the determination of guaninase, but also used as electrophoresis buffer. The working concentration is 3-100ml. Electrophoretic buffer is an important component of the gel electrophoresis system of nucleic acid and protein. It is also a conductor in the electrophoresis field and a necessary condition for maintaining the constant pH value of the electrophoresis system.
Buffer solution refers to the mixed solution composed of weak acid and its salt, weak base and its salt, which can offset and reduce the influence of strong acid or strong base on the pH value of solution to a certain extent, so as to keep the pH value of solution relatively stable. In biochemical research, buffer solution is often used to maintain the pH of the experimental system.
The change of pH value of solution system in research work often directly affects the effect of research work. If the pH value of the "extraction enzyme" experimental system changes or greatly changes, the enzyme activity will decline or even completely lose. Therefore, the preparation of buffer solution is an indispensable key step.
Buffer solution is an important concept in Inorganic Chemistry and analytical chemistry. Buffer solution is a solution that can maintain the relative stability of pH. In a certain range, the pH value does not change significantly due to dilution or addition of a small amount of acid or base. The buffer solution has different pH value and buffer capacity according to the amount of conjugated acid-base pairs and their substances.
Desheng technology will continue to develop and research advanced biochemical reagents to meet the needs of the development of global medical laboratory technology and strive for human health. The company customized a variety of ready to use solution, welcome to consult, pH can be customized.
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