MOPS, or 3-(N-morpholine) propane sulfonic acid, with CAS1132-61-2, is a kind of N-substituted amino sulfonic acid on morpholine amino group, which is usually used as amphoteric ion buffer with excellent performance, and also a buffer with very wide application and huge demand in good's buffer. It has a high water solubility and a buffer range of 6.5-7.9.
Physical and chemical properties of MOPS
English Name: 3-morpholinopropanesulfonic acid
Molecular formula: C7H15NO4S
Molecular weight: 209.26
Melting point: 277-282℃
Structural formula:
Different from the traditional weak acid salt buffer, MOPS does not participate in or interfere with the biochemical reaction process, does not denature or affect the enzyme activity, and does not inhibit the enzyme chemical reaction. Therefore, it can be used for the research of organelles and easily denatured, pH sensitive proteins and enzymes.
Configuration of MOPS buffer
(1) Weigh 41.8g of MOPS and dissolve in about 700ml of deionized water or DEPC treated water according to the experimental requirements;
(2) Use 2 M NaOH to adjust the pH value to 7.0;
(3) Add 1 M NaOAc 20mL and 0.5M EDTA (pH8.0) 20 mL treated by DEPC to the solution;
(4) Fix the volume to 1L, use 0.45um filter membrane to remove impurities, and store in dark at room temperature.
If sodium ion is to be removed in the experiment, KOH is used instead of NaOH, and accurate pH value is required. PH measurement can be used, and the proportion of mops solution and sodium hydroxide can be adjusted to adjust pH.
Application examples of MOPS
RNA was used to extract MOPS from 1% agarose modified gel. Melted DEPC water, MOPS and agarose, then used microwave oven, then placed it at room temperature for 60℃, then added 37% formaldehyde.
In addition, MOPS can also be used as buffer in Northern blot hybridization of RNA separation and membrane transfer, electrophoresis buffer in protein purification, forced medium components of bacteria, yeast and animal cells, DNA extraction and PCR diagnostic kit buffer. Desheng has rich experience in the R&D and production of mops and other biological buffers, and is committed to serving IVD related enterprises at home and abroad.
