According to news reports, autumn and winter is the season of rapid spread of the new coronavirus, and the epidemic may continue to spread rapidly in this winter and next spring. In the special period of epidemic prevention and control, rapid and efficient detection, diagnosis and isolation of virus infected patients are the key to overcome the epidemic.
New Coronavirus pneumonia was detected by real-time fluorescence RT-PCR kit and virus gene sequencing in two ways. Among them, nucleic acid detection (fluorescent PCR) has become the main means of new coronavirus detection because it can get the detection results faster, has greater flexibility and universality, and is more suitable for epidemic prevention and control and management.
The principle of New Coronavirus nucleic acid detection kit is basically: by extracting RNA from patient samples, and performing reverse transcription polymerase chain reaction (RT-PCR), amplifying the virus information in the sample to amplify it, and finally reading the signal in fluorescence. If the signal is positive after PCR, it can be said that there is virus in the sample (already infected), otherwise it means that there is no infection.
Affected by the epidemic, the rapid increase in the production capacity of nucleic acid detection kits has also brought a sharp increase in the demand for reagent raw materials. Overnight, the virus preservation solution has become a hot product. According to the published reports, the reagents used in the coronavirus nucleic acid detection kit include Tris, HEPES, Tris HC, guanidine hydrochloride, BSA, guanidine isothiocyanate, EDTA, edta-2na, etc.
Among them, Tris, Tris HC and HEPES are similar in their functions as buffers, which are used to form PCR reaction solution / RT-PCR reaction solution / thermostatic amplification buffer, etc.; BSA is mostly used as a stabilizer in the preservation solution and reaction solution of restriction enzyme or modified enzyme;
Guanidine hydrochloride and guanidine isothiocyanate are commonly used reagents for nucleic acid extraction. Guanidine hydrochloride is a strong inhibitor of nuclease, which is conducive to extracting complete RNA from RNase rich tissues. Guanidine isothiocyanate is a powerful protein denaturant, which can quickly separate nuclear protein from nucleic acid; EDTA and edta-2na are mainly used as chelating agents to prevent metal ions such as Mg and Ca from activating protease. In order to prevent the rapid degradation of RNA, ensure the integrity of RNA and improve the accuracy of detection, virus preservation solution plays an important role.
The novel coronavirus pneumonia and the virus preservation solution play an important role in the diagnosis of diseases. In the very period of epidemic prevention and control, it is necessary to diagnose the new crown pneumonia.
Desheng is a new scientific and technological enterprise with "in vitro diagnostic reagent raw materials", "blood vessel additive", "biological buffer" and "pharmaceutical intermediates" as the leading, integrating R & D, production, sales and service.
At present, Desheng can provide nearly 100 kinds of in vitro diagnostic reagent materials, including Tris, Tris HC, HEPES, EDTA, acridine ester and other reagents, as well as virus preservation solution, the core material for detection. The average daily production of Tris can reach 1 ton, hoping to provide basic raw materials for the development of new crown vaccine, neutralizing antibody and rapid immunodiagnostic reagent.
Contact Person: Miss. Ankiwang