Nowadays, with the improvement of living standards, people are paying more and more attention to their physical health status, and the demand for physical examinations is also increasing. During a physical examination, blood analysis is a necessary item to be checked. When carefully observing the blood collection vessels, it can be observed that each type of blood collection vessel cap has a different color. This is because different reagents are added to tubes of different colors, such as blood collection tubes with Anticoagulant, coagulant and no reagent.
At present, the widely used reagent is Ethylenediaminetetraacetic acid dipotassium salt, which is one of the Anticoagulant and can prevent blood coagulation. However, the basis for its effectiveness depends on performance, such as molecular weight, pH value, solubility, or main content. So next, let's introduce this aspect to everyone.
Properties of dipotassium Ethylenediaminetetraacetic acid
Its appearance is white crystalline powder, without a pungent odor, and is easily soluble in water. As the finished product is a powder, it is easy to absorb moisture, with an average molecular weight of 404.6 and a pH value of 4.8 ± 1 in a 5% aqueous solution. The solubility in water is ≥ 60%, and it is of analytical purity grade, with a main content of over 99%. Adding it as a solution to a test tube has strong stability and can effectively protect blood components from damage.
Anticoagulant mechanism of dipotassium salt of Ethylenediaminetetraacetic acid
Because it needs to be prepared into a solution when added to a vacuum test tube, Ethylenediaminetetraacetic acid and Ethylenediaminetetraacetic acid anions can be dissociated on this basis. They can effectively chelate calcium ions in the blood, control calcium ion reaction from the source, prevent internal and external coagulation process, so as to achieve the purpose of blood coagulation and inhibit platelet aggregation. At the same time, compared with other anticoagulants of the same type, Ethylenediaminetetraacetic acid dipotassium has the smallest effect on blood cell composition and high solubility, so it is also a rational Anticoagulant that is highly respected in medicine.
Determination of pH value, main content, and solubility
In addition to the molecular weight that can represent the overall mass of Ethylenediaminetetraacetic acid dipotassium salt, the pH value, main content and solubility are also important. The measurement method is as follows:
1. Determination of main content: weigh an appropriate amount of sample, add water and ammonium chloride solution to fully stir and dissolve, then add an appropriate amount of Eriochrome Black T indicator, titrate with Zinc chloride standard titration solution until the solution turns red and purple, and then calculate the content according to the calculation formula. Desheng Ethylenediaminetetraacetic acid dipotassium salt is a dihydrate, so the main content may be higher than 100, which is normal.
2. PH measurement: Dissolve an appropriate amount of the sample to be tested in pure water, stir at room temperature for about 5 minutes, and then use a pH meter to measure it.
3. Solubility measurement: Take an appropriate amount of the sample to be tested and dissolve it in pure water. Stir at room temperature for 5-10 minutes to observe if it is completely dissolved.
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