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Enzymatic determination of glucose by Daos (cas83777-30-4)

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Enzymatic determination of glucose by Daos (cas83777-30-4)

Daos, Chinese name n-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3, 5-dimethoxyaniline sodium salt, the finished product is white or light blue powder, CAS number is 83777-30-4, molecular formula is c13h20nnao6s, molecular weight is 341.36, Daos is a new kind of Trinder's reagent, with high water solubility, stable reaction, wide pH range, as an excellent color reagent is widely used in diagnostic detection and biochemical experiments.

 

The detection principle is: in the presence of hydrogen peroxide and peroxidase, the new Trinder's reagent reacts with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazolylsulfonylhydrazone (MBTH) to form a very stable purple or blue dye, and then the substrate concentration is determined by spectrophotometry.

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Daos chromogenic reagent

 

1、 The advantages of Daos in blood glucose measurement experiment are as follows

The determination of glucose in blood is an important test item in clinical medicine. At present, the commonly used detection method is glucose oxidase coupled with peroxidase method. This method uses God to catalyze the oxidation of glucose to gluconic acid and produce H2O2. H2O2 oxidizes colorless reduced chromogen under the catalysis of pod to produce colored oxidized pigment, and then calculates the content of glucose in the sample according to the color of oxidized chromogen.

 

The initial reaction of this method is specific, but the indicator reaction is nonspecific. The detection performance of this method changes with the different reduction chromogens in the indicator reaction. The common reduction chromogens are linaniline (which is rarely used because it is suspected to be carcinogenic), and the improved Trinder's reagent Daos is coupled with 4-aminoantipyrine (AAP) as the reducing chromogen The maximum absorption wavelength of the oxidized pigment formed by the oxidation and condensation of Daos and AAP is 592nm. Under this wavelength, bilirubin and other substances in blood do not interfere with the absorption, which can reduce the absorption interference of bilirubin and other interfering substances on blood glucose detection.

 

Therefore, this method does not need to separate blood into serum, which is simpler and more accurate than the method widely used in clinical enzymatic analysis with phenol as the reduced chromogen. The linear range of glucose is 1 ~ 20mmol / L, and the recovery is 96.3% ~ 97.7%.

 

2、 Instruments and reagents:

UV VIS spectrophotometer, glucose oxidase, peroxidase, Daos, AAP, anhydrous glucose, citric acid and trisodium citrate dihydrate, glycerin, bovine serum albumin, blood glucose detection kit, God and pod were prepared with pH = 6 buffer solution, and other reagents were prepared with distilled water.

 

3、 Methods: the experiment was carried out

In 1cm cuvette, add 0.5ml (25.6u) of God, 0.5ml (11.5u) of pod, 0.1ml (1.7mmol / L) of Daos, 0.1ml (2.9mmol / L) of AAP and 0.8ml of distilled water in turn, then add 40ul (8.3mmol / L) of glucose solution, stir well and use distilled water as reference to determine the absorption spectrum.

 

Desheng biochemical focuses on the development and production of in vitro diagnostic reagent raw materials, mainly including blood collection additive, chemiluminescent reagent, color reagent, enzyme substrate, antigen antibody and other products. The products are widely sold in domestic and foreign markets. Desheng's goal is not to be big, but to be a strong person in the precise field of the industry and win the market trust with its own professional.

Pub Time : 2021-04-18 16:46:13 >> News list
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