Today we will introduce TRIS, HEPES, TAPS, the team products in the Good’s buffer produced by Desheng.
TRIS (Trishydroxymethylaminomethane):
Weakly alkaline, with a pKa of 8.1 at 25°C, and the effective buffer range of its buffer is between pH 7.0 and 9.2. It is often used as a buffer in electrophoresis and gel experiments, and is widely used as a solvent for nucleic acids and proteins. It can also be used for the formation of nematode intermediate fibers. The most common role in cosmetics is to adjust the PH value (pH). It can also be used as a neutralizer to neutralize a thickener (carbomer) to reduce stickiness, improve skin cell respiration efficiency, and prevent pore clogging . In addition, TRIS can be used as an odor regulator in cosmetics containing amine salts to adjust the amine odor generated by friction when applying cosmetics, so as to avoid any residual or sustained odor release.
HEPES (4-hydroxyethylpiperazine ethanesulfonic acid):
It is a zwitterionic biological buffer, belonging to Good’s buffer, with an effective buffer range of 6.8~8.2. It can be widely used in a variety of biochemical reactions and used as a buffer reagent in certain cell culture media. In addition, due to its non-cytotoxicity, HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits.
TAPS (Trimethylol methylamino propane sulfonic acid):
It is also a zwitterionic buffer. In biochemical experiment projects, it can be used as an important pH stabilizing reagent. It is generally mixed with weak acid and its conjugate base to obtain an appropriate pH value. TAPS reacts with most organisms under neutral conditions. The pH value is required to be selected in the range of 6 to 8. At the same time, the effective buffer range of the buffer should also be specified in the range of 6-8. Moreover, it is necessary to ensure that the pH form of the buffer reagent cannot chelate with certain metal chemical ions. In the field of clinical diagnosis, TAPS is also commonly used as a biological buffer. Usually used in some biochemical diagnostic kits, PCR diagnostic kits and DNA/RNA extraction kits and other kits.
