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Wuhan Desheng Biochemical Technology Co., Ltd
Wuhan Desheng Biochemical Technology Co., Ltd
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How to Prepare HEPES Buffer?

How to Prepare HEPES Buffer?

The Chinese name of HEPES is 4-hydroxyethylpiperazine ethanesulfonic acid, which is a biological buffer with a wide range of uses. Perhaps when it comes to biological buffers, the first thing that comes to mind is its application in biochemical experiments, which is far from our daily life. In fact, if you carefully observe the commonly used skin care products and cosmetics, you will find that there are 4 - Hydroxyethylpiperazineethanesulfonic acid in these ingredient.


Due to the special buffer range of HEPES, it has an irreplaceable position in the field of industry, cosmetics and biochemical experiments. So how is it produced? The editor summarizes a method for preparing HEPES here, let's take a look.


Preparation steps of HEPES powder:

1.Vinylsulfonic acid or vinylsulfonate and N-hydroxyethylpiperazine addition reaction to prepare 4-hydroxyethylpiperazine ethanesulfonate;

2.4-hydroxyethylpiperazine ethanesulfonic acid salt is converted into the salt of 4-hydroxyethylpiperazine ethanesulfonic acid and corresponding acidifying agent with acidifying agent, obtains acidifying liquor;

3.The acidified liquor is crystallized, and the salt content of the corresponding acidulant is removed to obtain the treated acidified liquor;

4. Add soluble barium salt or calcium salt to the acidified liquor, remove residual sulfate, evaporate and concentrate to obtain HEPES primary purified product;

5. The primary purified product is washed with small molecular alcohol and dried to obtain high-purity HEPES powder.


This method can not only produce high-purity HEPES, but also has low purification cost, simple preparation process, easy operation and no pollution. Desheng Biochemical is a source manufacturer of biological buffers. Since established in 2005, it has rich experience in the research and production of biological buffers. At present, there are more than ten kinds of biological buffers produced. Welcome to inquiry.