We generally know that luminol is a chemiluminescent reagent, which is generally used for blood testing. It is a yellow crystal or beige powder at room temperature. It is a strong acid and has a certain irritating effect on eyes, skin and respiratory tract. Under alkaline conditions, it can be oxidized by peroxides and emit light at the same time. The redox reaction between luminol and peroxide requires a catalyst. This catalyst is generally a multivalent metal ion, peroxidase such as iron and horseradish. Peroxidase, etc., this method is often used to detect the content of peroxides, heavy metals, peroxidase, and the derived free radicals, for toxicant analysis and analysis methods based on peroxidase and glucose oxidase .
Luminol Luminous Picture
It is our incomplete understanding of it that leads to many questions about him in many cases.
1. When luminol detects blood, can it distinguish between human and animal blood?
Answer: No, because luminol detects blood mainly using the catalysis of iron in hemoglobin, and animal hemoglobin is not much different from humans, so luminol can only detect blood and cannot distinguish what kind of blood it is. If you accidentally mix human blood with blood from other animals, it is impossible to determine whether it is human blood just with luminol. But luminol has a good advantage that it does not affect DNA changes, so as long as we can detect blood, we can distinguish between human blood and other blood in DNA comparison.
2. Will the blood be washed with detergent affect the detection of luminol?
Answer: If the criminal suspect takes some measures after committing the crime, such as cleaning the scene with hypochlorous acid bleach, as long as he sprays luminol on the crime scene that has been washed with hypochlorous acid, it will glow everywhere, which will interfere with blood stains. Detection. However, although both luminol and hypochlorous acid can make luminol emit light, the two luminescence phenomena are different. The luminescence caused by bleach flashes quickly, while the luminescence caused by blood stains appears gradually.
3. Can we continue to use luminol after one reaction?
Answer: The luminescence principle of luminol is because hemoglobin contains iron, and iron can catalyze the decomposition of hydrogen peroxide, which turns hydrogen peroxide into water and monooxygen, and monooxygen oxidizes luminol to make it glow. When we use luminol, it will not change and oxidize the iron ions in the blood, and iron ions can continue to play a catalytic role as a catalyst. Therefore, the light-emitting process can continue to react. So the second use of luminol can still see the light.
4. Is it toxic if it accidentally sticks to the hands when using luminol?
Answer: The skin absorbs luminol very poorly. Even if luminol enters the body through the skin or other means, it will not deposit in the body or cause chronic toxicity. Moreover, there has been no report of luminol poisoning caused by improper protection. It can be inferred from the above that even if luminol easily enters the blood through the mucosa of the gastrointestinal tract, it will be quickly excreted from the urine. The chance of luminol passing through the skin or depositing in the body is very small, thus causing chronic poisoning. The possibility is very small.
The above are a few common questions we have sorted out through customer feedback, and hope that we can really help you in the follow-up use. Desheng Technology has more than ten years of experience in the field of chemiluminescence reagents. In addition to luminol, we also have acridine esters and their derivatives, which have left a good reputation for customers. We will continue to do our products with heart.
