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How to choose blood anticoagulant sodium fluoride heparin sodium citrate EDTA

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How to choose blood anticoagulant sodium fluoride heparin sodium citrate EDTA

Sodium fluoride heparin sodium citrate EDTA is a commonly used additive for vacuum blood collection tubes, which is commonly used for blood anticoagulation. Although the current vacuum blood collection tubes work to a large extent by design and are generally considered to be routinely recommended practices, laboratory staff must be aware of the different applications of blood collection tube additives sodium fluoride heparin sodium citrate EDTA Be particular. Which of the sodium fluoride heparin sodium citrate EDTA is used as the blood collection tube additive is particularly important.


1) Sodium fluoride
Sodium fluoride inhibits the glycolytic enzyme enolase and is used to limit the in vitro consumption of glucose by cells in the collected blood samples. In fluorinated unseparated blood samples, since the upstream enzyme converts glucose into glucose 2-phosphate, glucose is still metabolized at a rate of about 5% to 7% per hour at room temperature. It may take 4 hours to completely inhibit glycolysis in a fluorine-containing test tube at room temperature. It should be noted that sodium fluoride may have enzyme inhibitory activity and is not suitable for enzyme immunoassay.

 

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2) Heparin Sodium Heparin Lithium
Heparin is a highly charged biomolecule-polymerized glycosaminoglycan. It can prevent blood clotting, but can be co-purified with nucleic acid according to the preparation method used. It is a well-known serum protein polymerase chain reaction inhibitor. If blood is collected for polymerase chain reaction analysis, it can be digested with heparinase.


3) EDTA and citrate
Both EDTA and citrate are used as anticoagulants. The use of different coagulants depends on the ongoing downstream medical/biochemical analysis-such as plasma enzyme levels, etc. EDTA is a complexing agent and polymerase chain reaction inhibitor because it can complex Mg2 + ions, but extraction procedures usually result in most of the EDTA being deleted. If you want to amplify from whole blood, citrate will effectively avoid this problem. If you think there is a problem with the inhibition of the sample polymerase chain reaction, please add a known polymerase chain reaction positive sample (such as a plasmid) with the extracted target to see if it diverges.


4) Summary
Blood collection tube additives are raw materials for biochemical reactions in medical equipment. Improper use by non-professionals or due to manufacturing-related problems, interference with test results related to blood collection tubes may have some impact on patient results, reduce laboratory efficiency, delay test results, and increase each time due to re-collection and re-testing The cost of testing. The optimization of blood collection tubes, the professional production capacity and standardization of suppliers are essential for reliable test analysis.


Desheng Biochemical provides product support for the entire industry chain of blood collection tube additives. Sodium fluoride heparin sodium citric acid EDTA is a conventional product of Desheng Biochemical. This reminds diagnostic companies and laboratory workers to be vigilant to prevent the adverse effects of blood collection tube problems on clinical laboratory determinations.

Pub Time : 2021-07-20 16:05:35 >> News list
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