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How to realize the detection method of insulin

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How to realize the detection method of insulin

As we all know, for patients with diabetes, in addition to fasting blood glucose and postprandial blood glucose, glycosylated hemoglobin, as a gold indicator of blood glucose control, can reflect the average level of blood glucose control in 2-3 months, and should be reviewed every two to three months. In addition, there are two tests are also very important, that is, patients with diabetes often need to go to the hospital for insulin and C peptide testing.

 

Insulin is a very important hormone for human body, it has a good effect in lowering blood sugar. Studies have shown that there are many methods to measure insulin, and the requirements of each method are different. So this article will take friends to understand the detection methods of insulin?

 

Insulin is a peptide hormone secreted from islet B cells. Insulin secretion is insufficient, which can cause diabetes. But with the continuous improvement of people's living standard, the development of medical level and the change of people's concept of health, the incidence rate of diabetes is increasing rapidly. It has become a chronic and serious disease that plagues many elderly people.

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At present, chemiluminescence immunoassay (CLIA), enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (R1a) are the most commonly used methods in clinic.

 

The following is a brief introduction to the basic detection principle of insulin. The function evaluation of islet B cells is usually achieved by measuring insulin in serum. In the radioimmunoassay experiment, excessive labeled antigen * Ag and unlabeled antigen Ag (i.e. the antigen to be tested) are added to competitively bind with a small amount of antibody (AB). If the radioactivity of the conjugate (* Ag AB) measured by the experimental results is higher, it means that the concentration of the substance to be measured is lower. If the radioactivity of the measured conjugate is low, it means that the concentration of the analyte is high. By analyzing the standard curve, the concentration of the analyte can be calculated.

 

In 1960, the rapid development of endocrine science fully proved the great impetus of radioimmunoassay. In 1977, the inventor of this technology won the Nobel Prize for biomedicine. Subsequently, this new technology quickly penetrated into other fields of medical science, such as virology, pharmacology, hematology, immunology, forensic medicine, oncology, etc. radioimmunoassay plays a great role in promoting the progress of biomedicine in China. Desheng biochemical has a lot of raw materials supporting immunoassay, such as chromogen substrate, chemiluminescence reagent, biological buffer, etc.

 

The above is the method of insulin detection, hope to help friends. In fact, friends in the detection of insulin or should be in the morning 7 ~ 11 o'clock, and take the morning of the first small make-up to the hospital. In the diet or should pay attention not to eat with more sugar food. Otherwise, we will see the results of insulin test. If so, what do not understand the place can also look for expert consultation

Pub Time : 2021-01-08 13:24:52 >> News list
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