Influence of dipotassium EDTA pollution on the detection of heparin blood biochemical items
Ethylenediaminetetraacetic acid (EDTA) is a commonly used anticoagulant for testing, there are three kinds of disodium (EDTA-Na2), dipotassium (EDTA-K2) and tripotassium (EDTA-K3).
Routine blood is a common blood test item. In order to ensure that blood does not coagulate when blood is drawn, blood collection tubes containing anticoagulant are currently used. The commonly used anticoagulant is EDTA dipotassium (EDTA-K2). That is, its anticoagulant effect is very good, and it has little effect on the shape of blood cells.
Because potassium salt is more soluble than sodium salt, EDTA-K2 and EDTA-K3 are better than EDTA-Na2; and because the pH of EDTA-K2 is lower than that of EDTA-K3, it can compensate for cell wrinkles caused by osmotic pressure. shrink, so come out.
EDTA-K2 pollution often causes abnormally high K concentration in patients, which is inconsistent with the clinical manifestations of patients. However, EDTA-K2 pollution may also affect other biochemical indicators, but how much EDTA-K2 concentration affects which conventional biochemical indicators What about the impact? This article by the lippi.g team in Biochem Med (Zagreb) systematically explores the effect of increased concentrations of adherent EDTA-K2 on the results of routine biochemical assays.
Materials and methods: This paper recruited 15 volunteers from the laboratory staff to be included in the study. Two vials of lithium heparin and one vial of EDTAK2 samples were collected from each study subject. EDTA whole blood was diluted with lithium heparin blood into samples containing lithium heparin at concentrations of 0%, 5%, 13%, 29% and 43% EDTA. Next, the samples were separated and tested for biochemical indicators: ALT, total bilirubin, total cholesterol, creatinine, iron, LDH, lipase, potassium, sodium, chloride, magnesium, phosphorus.
Results: As EDTA2K concentration (from 5% concentration) increased, calcium, cholesterol, iron, lactate dehydrogenase, magnesium test results decreased, but potassium increased. The EDTA concentrations that significantly increased the results of the sodium phosphate assay were 13% and 29%, respectively. With the increase of the concentration of EDTA, the results of ALT, total bilirubin and lipase did not change. Low concentrations of EDTA2K (from 5%) have greater effects on calcium, cholesterol, lactate dehydrogenase, magnesium and potassium. For sodium phosphorus iron, it is necessary to have a high EDTA2K (from 29%) to have an effect.
Hubei Xindesheng Material Technology Co., Ltd. is a relatively old brand of blood collection tube reagent manufacturers. The unit packaging volume of its anticoagulant EDTA dipotassium is 500g/bottle, and the shelf life is 3 years. It has served many domestic and foreign customers and has professional The R&D and production team of blood collection tube anticoagulants have accumulated 17 years of experience, rich product knowledge, stable product performance and good use effect. If you have any purchase needs, please feel free to call!
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