TOOS has a higher molar absorbance of the color product, which means that the sensitivity of the color reaction is higher, and it can be used for the determination of very small amounts of test items in the serum. The absorbance of the color reaction product formed by TOOS does not change much with time, that is, the color reaction product is stable and not easy to fade.
Precautions for using TOOS
TOOS is reducible. Although peroxidase is needed for detection, the enzyme is its catalytic function. Without enzyme, it may be slowly oxidized by oxygen in the air after being prepared into a solution. . The chromogen substrate needs to be prepared into a solution when used. The chromogen substrate produced by Desheng is a pure white powder, sealed and stored. After the solution is prepared, it should be prepared for immediate use. It will be slowly oxidized and discolored if it is in contact with air for a long time.
The absorption wavelength of chromogen MAOS is higher, reaching 630nm, which is less interfered by other substances in serum or other samples, and can be targeted for detection items that require high-precision values. However, the product of MAOS fades significantly, and the data needs to be observed in time during the test. Therefore, the MAOS test must be completed in time without interruption. Furthermore, the color reaction of MAOS has a wide adaptability to the pH of the reaction system, which greatly improves its adaptability. The reaction of some color reagents is often under acidic conditions, but biochemical detection usually requires the participation of enzymes. We know that the catalysis of enzymes is more sensitive to the pH of the reaction environment, and does not match the color reagents. Inactivation will be greatly restricted in use, but MAOS reagent does not have this problem.
Precautions for using MAOS
As MAOS is sensitive to light and humidity. Therefore, when we store, the storage temperature of refrigeration is between 0-10, and the temperature of frozen storage is below 0. If the storage temperature is not indicated, it is best to store in a cool place below 15 in principle. In principle, the expiration date of the product is because the reagents may change over time due to storage conditions or trace impurities mixed into it. Therefore, it is difficult to strictly determine the expiration date or shelf life.
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