Luminol and Acridine esters,Indispensable for Chemiluminescence Immunoassay
Compared with traditional techniques such as radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay (CLIA) is a new technology based on chemiluminescence (CL) reaction, which has attracted much attention in the past decade.Due to the associated wide dynamic range and high sensitivity, this technique quickly become the predominant method for clinical immunological analysis. It is widely used in tumor, infectious disease, metabolic disease and pregnancy detection.
There are three kinds of chemiluminescence immunoassay (CLIA).
First one is chemiluminescence immunoassay (CLIA)
Commonly used labeling substances are isoluminol, acridine esters, these labels can generate special luminescent groups and directly participate in the reaction during the analysis process.
Directly labeled chemiluminescence immunoassay with acridinium ester: After taking immune reaction with the corresponding antigen (antibody) in the sample to be tested, a solid-phase coated antibody-antigen acridinium ester-labeled antibody complex substance. The oxidant (H2O2) and NaOH make the solvent to be alkaline, and the acridine ester decomposes and glow without catalyst.Substances like luminol for Chemiluminescence immunoassay: The luminescence of these substances is oxidation reaction. In alkaline solution, luminol can be oxidized by many oxidants, among which hydrogen peroxide is the most commonly used. Due to the slow reaction of luminescence, some enzymes or inorganic catalysts need to be added. Enzymes are mainly horseradish peroxidase (HRP), inorganic types include ozone, halogen,Fe₃+,Cu₂+,Co₂ and their complexes.
Second is indirect luminescence immunoassay
Commonly used markers are horseradish peroxidase (HRP, the common substrate is luminol or its derivatives, such as isoluminol) and alkaline phosphatase (ALP, the common substrate is 1,2-dioxane Ethane derivatives, AMPPD), such markers act as catalysts or energy transfer receptors and participate for luminescence reactions.
Third one is electrochemiluminescence immunoassay technology
A commonly used marker is ruthenium terpyridine with tripropylamine as a commonly used substrate.
The three luminescence technologies have their own merits and are widely used in the diagnosis of different diseases.
Chemiluminescence immunoassay technology is of great significance in the field of clinical diagnosis, and chemiluminescence reagents play a huge role. The isoluminol and acridine ester series products produced by Desheng Biochemical have stable quality and are supported by excellent scientific researchers and after-sales teams. Welcome to inquire http://www.whdsbio.cn/product/13.html
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