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Wuhan Desheng Biochemical Technology Co., Ltd
Wuhan Desheng Biochemical Technology Co., Ltd
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Method for labeling nucleic acid with acridinium ester

Method for labeling nucleic acid with acridinium ester

As a chemiluminescent reagent, there are many different models of acridinium ester. Among them, there is an acridinium ester NSP-DMAE-NHS, which has a labeling effect on nucleic acids. I believe many people may not know it. We will introduce this Details of the acridine esters.


Acridinium ester NSP-DMAE-NHS, CAS number 194357-64-7, appearance is yellow powder, it is a very important chemiluminescence reagent. It has the advantages of mild reaction conditions, good reproducibility, high luminous efficiency, and strong luminous intensity. It is widely used in the fields of inorganic and organic compounds, environmental monitoring, biological and pharmaceutical analysis, and it is also widely used in sensitive detection of various types of diseases. And diagnosis.


In terms of in vitro diagnosis, acridinium ester compounds are very suitable for labeling DNA strands to produce chemiluminescent DNA probes. Therefore, a method of how to label nucleic acids with acridinium esters will be introduced below.


Nucleic acid is the most important substance in all biological molecules. It is widely present in all animal and plant cells and microorganisms. Nucleic acid is divided into two categories: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Modern medical research results show that many diseases such as cancer and genetic diseases are related to DNA mutations, and many infectious diseases are caused by viruses, germs or parasites in the environment. Therefore, analysis of virus specific sequence DNA Conducive to the control of the epidemic.


In nucleic acid hybridization analysis, the preparation of labeled probes with strong specificity and high sensitivity is the key to successful nucleic acid hybridization analysis. Acridinium ester derivatives can be directly labeled on nucleic acid probes without the need for catalysts and the luminescence quantum yield is not affected. In addition, under certain conditions, the labeled acridinium ester on the unhybridized single-stranded DNA is hydrolyzed and destroyed, and only the double-stranded protected acridinium ester formed by hybridization can produce chemiluminescence, and the entire hybridization process can be monitored without separation.


This method for labeling nucleic acids with acridinium esters mainly includes three steps. Firstly, the 5'and 3'ends of the DNA probes are protected respectively; then the acridinium ester labeling is carried out, and finally the DNA is purified and separated by HPLC. Among them, acridinium ester labeling is the most important. Dissolve 25mM acridinium ester in dimethyl sulfoxide (DMSO), configure 1M HEPES buffer (PH=8.0), and follow the molar ratio of nucleic acid probe: acridinium ester=1:5 Add to HEPES buffer and react at 37°C for 1h.


This method creatively labeled acridinium esters on both ends of the DNA, further enhancing the sensitivity of detection. At Hubei New Desheng Materials Co., Ltd., we have many years of experience in the production and development of acridinium esters. A lot of energy has been invested in the research and development of acridine esters. At present, the company's products have been sold to more than 100 countries around the world, and most of them have received good reviews for repurchase. The product quality is excellent and the price is favorable. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.