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Positioning in biological buffer

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Positioning in biological buffer

Buffer solution is a kind of solution which can resist the influence of a small amount of strong acid and alkali outside, and maintain the pH value of the system basically unchanged. It is of great significance in the research of biochemistry. We often use Tris, bicine, caps and so on, but often can't accurately understand the difference between them, for this kind of problem, you can take a look here.

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Packaging of Desheng buffer

 

Tris (trimethylolmethylaminomethane) is widely used in the preparation of buffer solution in biochemistry and molecular biology experiments. In biochemical experiments, both TAE and tbe buffers (for nucleic acid dissolution) need to use Desheng Tris. Because it contains amino groups, it can react with aldehydes. Its structural formula, pH value changes greatly with temperature. Generally, the pH value decreases by 0.03 for every degree of increase in temperature. The buffer characteristic of Tris is weak base, and its PKA is 8.1 at room temperature (25 ℃). According to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2.

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Bicine (n, n-dihydroxyethylglycine) is an important raw material of antibody detection reagent and nucleic acid detection reagent in the field of in vitro diagnosis. It is a kind of white crystalline powder. It is soluble in water, insoluble in acetone, DMF, DMSO, DMAC and other organic solvents. The pH buffer range is 7.6-9.0, and the pKa value is 8.3 at 25 ℃. It is also a widely used buffer. It is used in enzyme reaction buffer and electrophoresis buffer with working concentration of 3-100ml.

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Caps (3-cyclohexylaminopropanesulfonic acid) is mainly used in biochemical diagnosis kit, DNA / RNA extraction kit and PCR diagnostic kit. It is used as buffer for enzyme chemistry and HPLC separation of alkaline drugs. 3-cyclohexylaminopropanesulfonic acid (CAPS) can also support alkaline phosphatase activity and inhibit the growth of Aeromonas at pH 10.5, and can be dissolved in deionized water. After pH is adjusted to 11.0, caps can be used for the purification of fibronectin. Caps is also an important hydrophilic modifier, which can react with polyisocyanate under very mild reaction conditions and in the presence of suitable neutralizing amines, so as to prepare polyisocyanate products which can be emulsified into fine dispersion form in water and stored stably.

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When a small amount of strong acid or alkali is added into the buffer solution, the pH value of the solution changes little, but if the amount of acid and alkali is large, the buffer solution will lose its buffering effect. This shows that its buffering capacity is limited. The buffering capacity of buffer solution is related to the concentration of components. After 15 years of independent R & D and production, Desheng technology has been widely recognized and used by customers in China's local market. Over the years, many well-known domestic manufacturers have been choosing and using Desheng products. We can also customize the buffer with different pH value according to the customer's requirements.

Pub Time : 2020-12-09 13:31:06 >> News list
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