（1） Accurate weighing of reagents: select appropriate culture medium according to different fungi and uses, and the reagents required for the culture medium must be pure.
（2） Correction of pH value: put various components of the weighed culture medium into the container, mark and draw lines, heat and dissolve, supplement water, and determine the pH value. It is usually measured by a precision test paper or pH meter with pH of 6.8-8.0. Use 1N NaOH and 1N HCl to adjust the pH value to a suitable range.
（3） Filtration: put the glass funnel on the iron frame, then use gauze with cotton or filter paper to put it in the funnel, pour the above medium into it and filter until it is transparent.
（4） Subpackage: subpackage the filtered culture medium into the medium test tube or triangle bottle (5ml for each tube; 100ml to 150ml for each triangle bottle), pack the cotton plug and wrap it with kraft paper for sterilization.
（5） Sterilization: medium sterilization, commonly used high-pressure steam sterilization. Generally, the nutritional cells of microorganisms are killed when they are boiled in water, but the spores of bacteria have strong heat resistance, so they must be sterilized by high pressure steam to achieve the goal of thorough sterilization. According to the principle that the steam temperature increases with the pressure, that is, the higher the pressure, the higher the steam temperature. Therefore, under the same temperature, high pressure steam sterilization is better than dry heat sterilization. Moreover, in the case of damp heat, the protein is easy to coagulate and denature after the bacteria absorbs water, because the steam has strong penetration and good sterilization effect.
1. Samples of Hanks virus transportation medium should be detected when they are fresh. In case of bacterial contamination, the bacteria may contain endogenous HRP, and may also produce false positive reaction. If stored for a long time, it can polymerize in ELISA to deepen the background.
2. After the frozen sample is dissolved, the protein is partially concentrated and unevenly distributed. It should be fully mixed, gently and slowly, to avoid bubbles. It can be mixed upside down, and it should not be strongly shaken on the mixer.
3. Turbid or precipitated samples should be centrifuged or filtered first, and then tested after clarification.
Repeated freezing and thawing will reduce the titer of the protein, so if the sample to be tested needs to be preserved for multiple tests, it should be stored in a small amount of sub packed ice. There are some reasons for the straightness of the standard curve in ELISA: whether the preparation of the standard curve is improper, whether the hole washing part is sufficient, whether the reading is inaccurate or whether there is suction error. Find the reason and find the solution.
Due to the different raw materials and requirements, the storage of the medium is slightly different. The general culture medium is easy to be polluted or decomposed by bacteria after being heated and hygroscopic. Therefore, the general culture medium must be kept in a damp proof, dark and cool place. For some culture media (such as tissue culture media) that need strict sterilization, they must be stored in a refrigerator of 2~6℃ for a long time. Because the liquid culture medium is not easy to keep for a long time, it has been transformed into powder.
The Hanks virus transportation medium independently R&D by Desheng has been successfully put on the market, and customers in need are welcome to inquire for details.
Contact Person: Miss. Ankiwang