Heparin Sodium, also known as Heparin Sodium, is a common reagent that can be used as an in vitro anticoagulant or as an anticoagulant drug. As a sulfated polysaccharide, it plays an important role in the medical field, especially in preventing blood clotting. Below is a specific introduction to heparin sodium solution.
Nature and characteristics
The "heparin" in heparin sodium is a sulfated polysaccharide compound extracted from pig intestinal mucosa or bovine lung mucosa, which belongs to natural polysaccharide drugs. Its chemical structure contains multiple sugar chains, which carry negative charges, giving heparin strong anticoagulant ability. In vitro, due to its good solubility in deionized water, it is prepared into a solution and added to a test tube. After anticoagulation treatment of blood samples, it is suitable for various biochemical tests, such as liver and kidney function testing.
Preparation of heparin sodium solution
Before starting to prepare heparin sodium solution, the following tools and materials need to be prepared: electronic balance, volumetric flask, beaker, deionized water, glass rod, and washing bottle. Before operation, it is essential to maintain a clean working environment to avoid contamination of the solution with impurities, bacteria, etc.
1. Weighing heparin sodium: Use an electronic balance to accurately weigh the required proportion of heparin sodium raw powder. Due to the usually small dosage of heparin sodium, it is necessary to ensure that the weighing accuracy reaches 1/10000 to ensure the concentration and effectiveness of the subsequent solution.
2. Dissolve heparin sodium powder: Add the weighed heparin sodium powder to a beaker, and then add a certain amount of deionized water. Stir thoroughly with a glass rod until completely dissolved, paying attention to controlling the amount of deionized water added.
3. Transfer to a volumetric flask: Transfer the dissolved heparin sodium solution to the volumetric flask, paying attention to keeping the opening of the volumetric flask parallel to the eyes during the transfer process to avoid liquid errors.
4. Constant volume: After all the solution is dissolved, wait until the solution reaches the mark on the volumetric flask. During this process, pay attention to keeping the mark on the same level as the liquid level.
5. Mix well: Seal the volumetric flask and thoroughly invert to ensure the uniformity of the heparin sodium solution.
6. Check and confirm: Carefully check the weight of heparin sodium, volumetric flask volume, and other factors, and perform corresponding calculations to confirm the effective content and concentration of heparin sodium solution.
Preservation of heparin sodium solution
Normally, the prepared heparin sodium solution should be stored in a low-temperature environment of 0-4 ℃, which can help prolong the stability and effectiveness of the solution. At the same time, pay attention to the sealing of the storage container to prevent external air and pollutants from entering the solution, and maintain the solution free of impurities. The suggestion is to use it immediately, and if it is necessary to store it, it should not exceed one week.
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