When biochemical reactions are carried out, a constant pH value is usually required. A good buffer can keep the pH value constant and reach a stable working condition. In the process of cell culture, the growth and metabolism of cells will cause drastic changes in pH value of culture medium. In order to maintain the stable growth environment of cells, it is necessary to add appropriate amount of biological buffer in the culture medium.
Here is a brief introduction to how to maintain the pH value of biological buffer solution, because the buffer is composed of weak acid (HA) and its conjugated base (a –) or weak base and its conjugated acid. Weak acids and bases can not be completely dissociated in water, but exist in the equilibrium state of dissociated and non dissociated species in buffer solution.
For example, in acetic acid solution, there is an equilibrium of acetate ion, hydrogen ion and undivided acetic acid. Because of the dissociation of acetic acid (HAC), it can absorb H + or Oh -. HAC can release H + to neutralize Oh - and form water. Conjugated base a – can react with H + ions added to the system to produce acetic acid.
There are so many biological buffers and alternatives that can be used in cell culture on the market. Why choose HEPES? In fact, the suitable culture environment for most cells is ph7.2-ph7.4, while the application range of Desheng HEPES buffer just falls within ph6.8-ph8.2, which is in line with the characteristics of pH value needed for cell culture.
Compared with other buffers, HEPES has higher stability in maintaining the pH value of cell culture medium. Therefore, HEPES buffer is widely used in cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell division, living cell imaging and other biological and biochemical research.
Desheng is a professional biological buffer manufacturer with strong logistics capacity and rich export experience. We produce high-quality and high-purity HEPES buffer. We can provide a full range of product testing and special testing services according to your application needs.
In addition, we can also provide a variety of packaging specifications, HEPES purity ≥ 99%, good water solubility, stable process, can control the constant pH range for a long time. In any case, Desheng will be the best choice for you to buy HEPES!
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