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Simple Introduction for MOPS Buffer

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Simple Introduction for MOPS Buffer
Latest company news about Simple Introduction for MOPS Buffer

The full chemical name of MOPS is 3-morpholinepropanesulfonic acid. It is a white crystalline powder at room temperature with good hydrophilicity. 10g of MOPS powder can be completely dissolved in 100ml of water at 20°C, and the dissolved solution is colorless and transparent.MOPS is often prepared as a zwitterionic biological buffer to adjust the pH of the solution in biochemical reactions.

 

Specifications of MOPS:

CAS No.:1132-61-2

Molecular Formula: C7H15NO4S

Molecular Weight:209.3g/mol

PH range:6.5-7.9

pKa(25℃):7.0-7.4

Purity:>=99%

 

As a common buffer, MOPS buffer has a wide range of applications.Editor of Desheng will elaborate on several applications of MOPS buffers as following:

1. Since the pH range is between 6.5-7.9, MOPS buffer can be used to separate, purify, and extract RNA and protein, but cannot be used to separate DNA because it can interact with DNA and form complexes.

2. MOPS buffer can bind to iron, but does not react with most metals such as magnesium, manganese, cobalt, nickel, etc. Therefore,it is often added to stabilize the acidity of the solution in cell culture media that do not require iron ions, such as bacteria, charcoal yeast, and mammalian cells.It should be noted that the concentration of the buffer has a great influence on the PH of the solution, so when using the MOPS buffer to adjust the PH of the cell culture, the concentration of MOPS should be less than 20 mm.

3. In the non-denaturing agarose gel electrophoresis experiment of RNA, MOPS buffer is more suitable for stabilizing the pH value of the solution than other buffers.

4. Because the UV absorbance of MOPS buffer is very small, it does not interfere with the measurement of absorption during UV/Vis spectrophotometry.

5.At room temperature, the chemical properties of MOPS buffer are very stable. When it interacts with the peptide backbone of bovine serum albumin, it can promote the protein to reach a static stable state.

 

MOPS buffer has a strong buffering capacity in the pH range of 6.5-7.9. The point should be noticed is that MOPS can change the interaction between lipids, such as affecting the thickness and barrier properties of the rat endothelial surface layer, the mRNA expression form of bovine embryos produced in vitro, etc.It can be oxidized by strong acids and strong alkali.Therefore,these substances should be avoided in application. Hubei New Desheng Material Technology Co., Ltd. is a professional manufacturer of biological buffers. The production process is stable and the appearance of the product is pure white crystal powder. Welcome to consult.

Pub Time : 2022-05-11 09:50:14 >> News list
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