Heparin sodium, as a commonly used anticoagulant, is often added to blood collection vessels to ensure that blood remains in a liquid state during collection, transportation, and analysis, preventing coagulation. This additive with bioactive molecules requires a specific concentration of solution to ensure the anticoagulant effect in the test tube. However, as the original form of heparin sodium is powder, it needs to be prepared into a solution in advance before use, and there are certain requirements for its solubility. Therefore, let's take a look at the solubility of heparin sodium together.
Heparin sodium powder
Solubility of heparin sodium
The raw material of heparin sodium is powder, which has a certain solubility in water. Its solubility is often expressed as a percentage, in the form of w/v (mass/concentration). Normally, a 5% (W/V; 5g/100ml) heparin sodium solution is clear, which means that every 100 milliliters of solution contains 5 grams of heparin sodium. This concentration is sufficient to provide the required anticoagulant effect in the blood collection vessel, but sometimes it may be adjusted according to the needs of the enterprise.
Factors limiting the dissolution of heparin sodium
The dissolution of heparin sodium is sometimes influenced by factors such as temperature, pH value, and ion strength. Under normal room temperature conditions, powder is easily soluble, but under extreme conditions such as high or low temperatures, its solubility is limited. In addition, the pH value of heparin sodium in a 1% (W/V; 1g/100ml) pure water solution is between 6.0 and 8.0. If the dissolution pH value is too high, it will also cause changes.
Usage and dosage of heparin sodium in blood vessels
1. Solution preparation: In order to add heparin sodium to the blood collection vessel, it is first necessary to prepare a suitable concentration of heparin sodium solution. Usually, a ratio of 1:100 is used, which involves adding 1g of heparin sodium to 100g of deionized water, but this is not fixed and can be adjusted according to usage requirements.
2. Dosage selection: The dosage of heparin anticoagulation is usually determined based on the specific needs of experiments or blood collection. Generally speaking, the recommended range for heparin anticoagulation is 9.4-28IU/ml blood sample. According to the volume of the blood sample and the required degree of anticoagulation, an appropriate dosage of heparin sodium can be selected. For example, usually 20 IU of heparin anticoagulant 1 mL of blood sample is selected, or the dosage of 0.1 mg of heparin anticoagulant 1 mL of blood sample is prepared.
Heparin sodium is a commonly used additive in blood collection, and a thorough understanding of its solubility and dosage is of great help in ensuring experimental results. As an advantageous manufacturer of heparin sodium, Desheng can provide raw material powders with good solubility and cheap prices, as well as technical guidance and instructions, effectively solving customer usage problems. If you have any interest, please feel free to contact us for purchase!
