Company News About The difference between acridinium ester and other mainstream chemiluminescent reagents
Acridinium ester, a type of chemical substance that can be used as a chemiluminescent marker, with a yellow powder appearance. In alkaline H2O2 solution, the molecules of acridinium ester are affected by hydrogen peroxide ions. The ring C-9 and hydrogen peroxide form unstable dioxane, which decomposes into CO2 and electron-excited N-methylacridone.
Acridinium ester reaction process
The acridinium ester chemiluminescence process reacts quickly and has a low background, and can emit light in the presence of sodium hydroxide and hydrogen peroxide. During the oxidation reaction, the conjugate is decomposed without affecting the luminescence of the free acridinium ester; in addition, the acridinium ester chemiluminescent reagent has good stability and is easy to store.
The difference between acridinium ester and other mainstream chemiluminescence reagent
| Type | Marking, reaction system | Main advantages | Main disadvantages | |
|
Chemiluminescence |
Enzymatic | Peroxidase, luminol, oxidant, enhancer, etc. | Simple measurement method (rate method), low cost, high sensitivity | The working curve drifts with time and the low-end slope shifts nonlinearly |
| Spiral adamantane epoxide, alkaline phosphatase | Simple measurement method (rate method), high sensitivity | |||
| Non-enzymatic | Acridinium ester, hydrogen peroxide | high sensitivity | Short luminescence time, complicated measurement method (integral method), in-situ injection (In Situ Injector), high instrument cost and maintenance cost, and high reagent cost | |
| Electrochemiluminescence | Pulsed electric field excitation of ruthenium (Ru) complex | High sensitivity and stable reagents | Complex measurement methods (pulse excitation, intermittent measurement, flow cell), high instrument cost and maintenance costs, similar elements in the environment and samples can lead to background interference | |
Desheng Technology develops and produces 6 acridinium ester products with different groups. Although the substituents are different, they have the same acridinium ring. Whether they are acridinium ester or acridinium hydrazide, their luminescence principles are the same. The acridinium ester developed and produced by Desheng Technology belongs to laboratory production, and the quality control is stricter. Desheng has a professional technical R&D team, an excellent logistics partner, professional packaging services, and more than ten years of production R & D experience. It has the ability to provide end users with a strong problem-solving ability. Looking forward to you further consultation. Please contact tel:0711-3702650 for details.
