The influence of heparin salt represented by heparin sodium and heparin lithium on blood test

Heparin salt anticoagulant represented by heparin sodium and heparin lithium is a commonly used additive for blood collection tubes. It plays an important role in the big family of blood collection tubes. In this analysis of blood collection tube additives, we will analyze the effect of heparin sodium and heparin lithium heparin salt on blood clinical testing under the condition of other factors being fixed. These factors such as the components in the blood collection tube, including the choice of anticoagulant, surfactants and separating gels, plugs, lubricants, the design of blood collection tubes or the use of blood collection equipment, etc. Let alone study the influencing factors of the anticoagulant heparin sodium and lithium heparin.

Figure-Lithium heparin produced by Desheng Biochemical is ready to go
It is well known that serum is used in most assays, but plasma is a useful and efficient alternative detection method due to its short processing time. Plasma containing fibrinogen and other clotting factors has a higher viscosity and total protein content than serum. The serum levels of hemagglutinin, potassium, coagulation factor activating peptide, platelet factor and platelet components released during platelet activation are high. When using plasma, the anticoagulant used to preserve the analyte may interfere with the determination of other analytes.

Heparin salts (usually from porcine intestinal mucosa) are also widely used as anticoagulants in blood collection tubes. Heparin forms a complex with antithrombin III and induces its conformational changes to accelerate the inhibition of thrombin factor, thereby preventing thrombin activation and fibrin production from fibrinogen. Heparin binds electrolytes and changes the concentration of bound ions and free ions to create an electrolyte balance formula. Heparin and citrate are the most commonly used anticoagulants. Heparin interferes with the chloride electrode membrane, creating a negative anion gap in the specimen for medical diagnosis.

Heparin slows down the speed of certain antibody-antigen reactions, especially in the precipitation step in the secondary antibody system. Heparin precipitates cryofibrinogen in cryoglobulin studies. Exogenously administered heparin changes serum thyroid hormone levels. When using heparinized test tubes in hemodialysis patients, falsely low albumin levels were observed. Heparin can inhibit the binding of bromocresol green to albumin, leading to the formation of colorimetric complexes and achieving better anticoagulant effects faster.

Proteomics studies have shown that heparinized plasma can cause non-specific protein binding, which affects peptide separation and mass spectrometry. Incomplete filling of lithium heparin tubes will produce higher creatine kinase and γ-glutamyltransferase activities.

Desheng Biochemical has been producing heparin sodium and heparin lithium for more than ten years. Heparin salt based on heparin sodium and heparin lithium is an ideal anticoagulant for hospitals and physical examination institutions. Desheng Biochemical has an eight-person research team composed of doctoral and master students to help the development of blood testing.