3-Morpholinepropanesulfonic acid is abbreviated as MOPS, CAS No. is 1132-61-2.It is a zwitterionic buffer commonly used in biochemistry and molecular biology, and its structure is similar to 2-(N-morpholine)ethanesulfonic acid (MES), also one of the "Good" buffers classified by Dr. Norman Good and colleagues in 1966. It has a wide range of applications, such as for mammalian cell culture (recommended concentration should be ≤20 mM), RNA denaturing agarose gel electrophoresis experiments (20 mM concentration is better for formaldehyde gels), discontinuous Polyacrylamide gel electrophoresis experiments, etc.
PH stability of 3-morpholinopropanesulfonic acid (MOPS)
The PH stability of 3-morpholinepropanesulfonic acid and its effect on vascular tension in vitro were studied experimentally. The results showed that the PH changes of MOPS buffer and K+-MOPS solution within 14 days were basically constant at (7.32±0.04)～(7.51±0.04) 0.04). The aeration rate had little effect on the pH of MOPS solution, and the range of change was (7.24±0.04)～(7.39±0.00).While the Krebs solution was sensitive to the aeration speed, and the pH range was (6.81±0.81±0.00) between 15 and 60 bubbles/min. 0.07)～(8.33±0.02), the fluctuation range is large.
Changes in the volume of MOPS solution and Krebs solution under the same ventilation rate have little effect on their pH. The isolated rat mesenteric artery, cerebral basilar artery and coronary artery can produce good contraction in MOPS solution. Therefore the MOPS solution has the advantages of stable pH, long standing time, easy ventilation, and low application cost, and can be used in mammals experiments on isolated tissues and organs such as blood vessels.
Precautions for the use of 3-morpholinopropanesulfonic acid (MOPS)
1. MOPS can interfere with Folin protein analysis, and autoclaving of MOPS solution containing glucose will cause its partial inactivation.
2. MOPS powder can be stored at room temperature for several years. However, upon standing, the bulk powder may clump together and form hard lumps without affecting its chemical properties.
3. Storage method: MOPS powder can be stored stably at room temperature for several years. Prolonged storage may clump without affecting its use and chemical properties.
4. MOPS buffer can be stored at 2~8°C for at least 6 months, and sterilized by filtration with a 0.2 μm filter. Autoclaving is not recommended. To prepare a nuclease-free MOPS solution, autoclave the water before adding the powder to dissolve.
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