There are many kinds of blood anticoagulants in blood collection tube reagents, and one anticoagulant has a wide range of uses, that is, potassium EDTA. There are many types of EDTA potassium salt, among which dipotassium is used more, but what about its principle and application? There should be some people who are not very familiar with it. Here we can take a look at what application areas and principles it has.
EDTA dipotassium salt is also known as dipotassium ethylenediaminetetraacetate dihydrate (EDTA-K2). It is a white crystalline powder, easily soluble in water, easy to absorb moisture, and has a molecular weight of 404.6. DETA dipotassium salt can protect the cell components of the blood, does not affect white blood cells, has the least impact on red blood cells, and can inhibit the aggregation of platelets, which is suitable for general blood tests. But for the separation test with platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor is it suitable for calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine amino peptide Enzyme determination and PCR test.
The dipotassium EDTA is mostly used in complexing metal ions and separating metals, as well as washing powder, shampoo, liquid soap, agricultural chemical spray, blood anticoagulant and other fields. The dipotassium EDTA is also recommended by the International Committee for Standardization in Hematology as the preferred anticoagulant for blood count and classification.
The principle of EDTA dipotassium salt as an anticoagulant, first of all, we first understand the principle of blood coagulation, which can be divided into three parts, 1. The formation of prothrombin activator, 2. The role of prothrombin activator in calcium ion With participation, prothrombin is converted into active thrombin. 3. Soluble fibrinogen is converted into insoluble fibrin under the action of thrombin. Fibrin is shaped like filaments, criss-crossed, and collects a large number of blood cells to form a jelly-like blood clot. This is how coagulation occurs. The dipotassium EDTA has a great affinity for calcium ions in the blood, and can complex with calcium ions so that prothrombin cannot be converted into thrombin, thereby making the blood anticoagulant.
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