The principle of Trinder's reaction is also known as enzymatic method.Under the action of peroxidase, hydrogen peroxide, 4-aminotipyrine (4-AAP), a red quinoneimine compound generates for the determination of light absorption,which is one of in vitro diagnostic reagent method.It is mostly used in biochemical diagnostic reagents to detect liver function, kidney function, blood sugar, blood lipids, etc.
Trinder's reaction is to mix the corresponding new Trinder's reagent, 4-AAP, oxidase and peroxidase to the item to be tested in an appropriate ratio, then add biological buffer and the test substance to cause a color reaction.The value of the item to be measured is calculated by measuring the absorbance of the red quinoneimine compound in the color reaction product. The enzyme in the reaction has specificity and can catalyze the reaction of a specific substance in a targeted manner, ensuring the accuracy of the detection. The biological buffer ensures that the reaction is carried out in the appropriate PH value of the corresponding enzyme.
Traditional Trinder's reagents are phenols (phenol, 4-chlorophenol or sodium 2,4-dichloro-6-phenolsulfonate, etc.) and anilines (N,N-dialkylaniline, N,N-Dialkyl-m-toluidine,etc). Phenols have a small absorption wavelength range, and the substances in the test may interfere with the test results. Aniline chromogen substrates need to be carried out under acidic conditions, which limits the use of enzymes, so they cannot be widely used in practical detection.
Compared with the traditional Trinder's reagent, the new Trinder's reagent has higher water solubility, a wider range of ultraviolet absorption waves of the color reaction product, a wider range of acid-base reaction requirements, and a more sensitive reaction, so it is more practical in biochemical reactions. There are 9 kinds of new Trinder’s reagents independently developed by Desheng, TOOS, TOPS, ADOS, ADPS, ALPS, DAOS, HDAOS, MADB, MAOS, all of which are white crystalline powders with a purity of over 99%. If the content of the item to be tested is little in serum, ALPS, TOOS, TOPS should be used because they are more sensitive to color; if accurate detection is required, MADB, MAOS can be selected, which have higher absorption wavelengths and not easily interfered by other substances; TOOS, ADOS, DAOS color reaction products are stable and not easy to fade; MAOS is suitable for the determination of wavelengths between 550nm-600nm.
Desheng is not only a high-quality manufacturer of the above-mentioned new Trinder’s reagents, our company can also provide raw materials for biological buffers, which provides convenience for your one-stop shopping with more competitive prices. Welcome to inquiry.
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