Luminol, also known as luminol. The chemical name is 3-amino-phthalic hydrazide. It is a pale yellow powder at room temperature, which is a relatively stable synthetic organic compound. Luminol is a strong acid, which has a certain irritating effect on eyes, skin and respiratory tract. Luminol is a chemiluminescent reagent, which can be converted into excited amino phthalic acid in the presence of hydrogen peroxide molecules, and then emits strong fluorescence.
Under normal circumstances, the chemiluminescence reaction of luminol and hydrogen peroxide is quite slow, but when there is a catalyst, the reaction is very rapid. The most commonly used catalysts are metal ions. Within a wide range of concentrations, the concentration of metal ions is proportional to the luminescence intensity, which enables chemiluminescence analysis of certain metal ions. This reaction can be used to analyze organic compounds containing metal ions. Achieve high sensitivity.
The second is to use certain compounds to determine the enhancement and inhibition of luminol on chemiluminescence. The initial application of luminol reagent was to detect proteins. In western blotting, an enzyme-linked antibody is often used to bind the protein to be detected, and the combination of luminol chemiluminescence reagent and enzyme (peroxidase) can make the local luminescence and reduce the protein The position is displayed. The luminescence in the picture is very bright, probably because of the chemiluminescence enhancer added to the reagent. Of course, the luminescence is far less bright when actually detecting proteins.
In addition, luminol derivatives such as isoluminol (ABEI) are labeled on carboxylic acid and ammonia compounds, and then separated by high performance liquid chromatography (HPLC) or liquid chromatography (LC), and then under alkaline conditions It reacts with hydrogen peroxide-potassium ferricyanide to perform chemiluminescence detection, such as labeling the newly synthesized chemiluminescence reagent isoluminol isothiocyanate to yeast RNA, separating it by centrifugation and dialysis, and then performing chemiluminescence detection.
Desheng's current chemiluminescence reagents include acridinium ester, luminol, etc., and the quality is guaranteed and the supply is sufficient.
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