At present, the domestic chemiluminescence immunoassay technology has been advancing by leaps and bounds, and is gradually in line with the level of international developed countries. Among them, chemiluminescence reagents are mainly developed around acridinium ester luminescence reagents and luminol reagents, so who will become the core C position of chemiluminescence reagents What?
Acridine ester chemiluminescence reagent
The difference between luminol and acridine esters:
1. Acridine esters and luminol are both very widely used luminescent reagents in the chemiluminescence immunoassay CLIA. There is a big difference between the two. Compared with luminol, the most intuitive thing is the sensitivity. Much higher.
2. The price of acridine esters is much higher than that of luminol. The price of acridine esters luminescent reagents is usually priced in milligrams or grams, with an average of several hundred yuan per milligram; while luminol is priced in grams or kilograms, gram price It ranges from tens to hundreds, so the spread is still relatively large.
3. Luminol, isoluminol and their derivatives are the earliest types of chemiluminescent substances used, which require the use of catalyst peroxidase POD and enhancers, which will increase the background luminescence and increase the measurement background. This limits the sensitivity of this technology and its application and development.
4. Acridine ester has high luminous efficiency, simple luminous system, no need to add catalyst, low background, simple marking. Because the thermal stability of the traditional acridinium ester AE-NHS is not very good, after that, the acridinium ester derivative which is more stable than AE-NHS has been synthesized and applied to CLIA. For example, DMAE-NHS has been proven to have good thermal stability and luminescence properties.
The reaction sensitivity of acridine-based luminescent reagents is very high. The addition of the excitation solution causes the reaction system to immediately release photons of about 430nm. The protein concentration can be detected by counting the number of photons with a standard luminometer. Because this light-emitting process is very short (the whole process is completed within 2 seconds), the sample must be placed directly in front of the photon detector inside the photometer. Proteins, peptides, antibodies, and nucleic acids can all be labeled with acridinium esters. The labeled compound emits light rapidly under the excitation of basic hydrogen peroxide, and the labeled compound can be detected by collecting photons.
Acridine esters are obviously superior to luminol in all aspects of CLIA, but its price and equipment cost are higher. In some cases where the detection requirements are relatively low, it is not necessary to use acridine esters.
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