In terms of detection, the higher the sensitivity, the better. The higher the purity of the reagent, the better the detection effect will be? The more expensive the reagent, the better the effect? Actually not. For diagnostic reagents, choosing the right reagent is the most important.
Does the higher the sensitivity of the reagent mean the best effect?
Not entirely, usually the higher the sensitivity means that the analyte with a very small content can also be detected, but the detection index content in the sample to be tested is high, and there is no need to require high sensitivity of the reagent; if the composition of the sample to be tested is complex , Reagents with high sensitivity may also detect some interfering substances, resulting in inaccurate final data. Therefore, in biochemical detection, in order to eliminate interference, MAOS will be used as the chromogen substrate; for the low content analyte, TOOS with high sensitivity is used as the chromogen.
Is the purity of the reagent the better?
No. Generally speaking, when purchasing diagnostic reagents, you usually pay attention to the purity data on the test report. The higher the purity, the better the quality. But for a specific experiment, the higher the purity, the better. Such as the simplest reagents, deionized water and ultrapure water. Some biochemical experiments can use deionized water. If ultrapure water is used, it means that all the reagents involved use ultrapure water, which increases the cost and the experiment operation becomes cumbersome.
As long as the reagent purity and impurity ion content can meet the needs of the experiment, the experiment can be carried out normally. It is not necessary to use high-purity reagent grade Tris for industrial synthesis of Tris, and injection grade heparin is not necessary for heparin used for anticoagulation. The purification cost of some reagents is relatively high, and the performance of some reagents can be improved by adding a small amount of other substances.
In vitro diagnostic reagents are used for scientific experiments in biochemical testing. It is a very rigorous work. The applied reagents need to be paid attention to, otherwise the test results will lose the significance of testing if they are inaccurate. Therefore, in the selection of reagents, it is necessary to choose the most suitable one to achieve the best results.
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