Tris powder sticks together during the drying and crystallization process, can it still be used?

The full name of Tris is tris(hydroxymethyl)aminomethane. It is a widely used biological buffer. Its hydrochloride solution is not only widely used as a solvent for nucleic acids and proteins, such as TAE and TBE buffers, especially nucleic acid solutions. . It contains primary amines, so reactions related to typical amines occur, such as aldehydes. Tris also complexes with metal ions in the solution.

As a domestic tris manufacturer, Desheng Biotech produces high-purity white crystals in powdered tris. Recently, I received a call from a customer and reported the high temperature situation. The viscosity of the product increased significantly around 126 degrees. The originally dispersed powder gathered into fine particles, and the reaction was carried out in an anhydrous dry reactor, basically eliminating the influence of moisture. Will this change affect product quality?

In fact, this involves the effect of temperature on the crystal structure of Tris. The molecular crystal structure responds to changes in externally applied conditions (such as temperature and pressure) in a complex balance between strong intramolecular bonding, moderate intermolecular interactions (such as hydrogen bonds) and weaker intermolecular van der Waals contacts result. Usually we also consider the influence of pressure on the crystal morphology. We have passed the autoclave test. This pressure above 75Mpa obviously affects the crystal shape. The additional thermodynamic requirements for effective space filling become more and more important. However, the pressure of the autoclave usually does not exceed 20Mpa, so the influence of pressure is not discussed here.

Our measurements are all performed using neutron powder diffraction. The characteristic of the crystal structure is the van der Waals interaction between the molecular chain formed by the NH...NH bond. Although hydrogen bonding is the strongest intermolecular contact, most of the sublimation enthalpy may be due to weaker but numerous van der Waals interactions. Analysis of the thermal expansion data based on the phonon frequency determined in the periodic DFT calculation shows that the expansion at very low temperatures is controlled by the external lattice mode, but above 126°C, the influence of the deflection mode of the intramolecular ring also changes Significantly. The main direction of thermal expansion depends on the sensitivity of different van der Waals interactions to distance changes.

The main value of strain generated when pressure is applied is similar to the value determined in the variable temperature study, but it is isotropic because it needs to fill the void under high pressure to minimize the volume. When the temperature is lowered to room temperature, the hydrogen bond between the molecules weakens, the H interaction between the molecules, the interaction between the dipole moments makes the force between the molecules weaker, and the tris presents a loose white powder structure. This is what we are. The shape of the finished product usually seen. The above changes are all physical changes, which are instantaneous changes in the polarity between molecules and do not change the chemical properties of the molecules themselves, so there is no product quality problem. You can use it with confidence.

Desheng Biochemical is a high-tech enterprise focusing on the fields of life science and biotechnology, providing Chinese experts and scholars with a full range of high-quality products. The tris, Tris-HCL, Bicine, CAPS, MOPS, TAPSHEPES, etc. produced have been widely used in life science basic research, development and application, biotechnology and many other fields. Customers are located in universities, research institutes, hospitals, biotechnology companies and food industry units.