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Tris buffer usage

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Tris buffer usage
Latest company news about Tris buffer usage

Tris is a zwitterionic buffer, often used in biology and scientific research experiments, of course, there are also many directly used in medicine and industry. So what are the specific uses of the different buffers of tris, how to use the buffers of tris, and understand the characteristics of tris and different biotechnologies, such as electrophoresis or chromatography.

 

Basic information of TRIS:

CAS number: 77-86-1

Molecular weight: 121.14 g / mol

Formula: C 4 H 11 NO 3

Available pH range: 7.2-9.0

pKa (25°C): 8.06

Maximum wavelength (λmax)λ: 260 nm Amax: 0.10: λ: 280 nm Amax: 0.08

 

latest company news about Tris buffer usage  0

What is TRIS recommended for?

lCommonly used in gel electrophoresis buffer (TAE or TBE)

lUsually used to prepare Laemmli buffer (used for denaturation and loading of protein samples in SDS-PAGE)

lAnion exchange chromatography

lUsed to evaluate bacterial endotoxin

lFor capillary electrochromatography (CEC), due to its low ion mobility

lUsed for metal complexation

l Can be used to correct acidosis

latest company news about Tris buffer usage  1

What issues should be considered before choosing TRIS for research?

uNot very effective below pH 7.5

uIt has potentially reactive primary amines and can usually be used as an inhibitor

uIt tends to permeate the membrane, so it is not suitable for most cell culture work

uToxic to many mammalian cells

uIt is not suitable for bicinchoninic acid (BCA) analysis

uIt may interfere with the Bradford dye binding method for protein determination

uChelated calcium and other essential metals are well known

uThe pH of the solution containing Tris is very dependent on temperature, so it is very important to prepare Tris buffer at the temperature of use

uIt may interact with the bicarbonate/CO2 buffer system

According to the use of different industries, the considerations are different, and the requirements for the level of tris will also be different, the purity and some other indicators will also be relatively different, and these factors often require buyers and manufacturers to be able to deepen Communication can be better provided.

 

Tips about TRIS:

ØHigh solubility in organic solvents

ØIt reacts with various enzymes and inhibits several enzymes

Ø It can inactivate the popular RNase inhibitor DEPC

Pub Time : 2020-11-02 16:00:00 >> News list
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