With the increasing demand for nucleic acid testing projects, Virus Transport Media’s procurement needs are also getting higher and higher. The ingredients of Virus Transport Media are: Hanks Liquid Foundation, Gentamicin, Fungal Antibiotics, BSA (V), Cryoprotectants , Biological buffers and amino acids, etc.
Virus Transport Media is suitable for the collection, preservation and transportation of common virus samples such as new coronavirus, influenza virus, hand, foot and mouth virus. Pharyngeal swabs, nasal swabs or tissue samples of specific parts can be collected, and the stored samples can be used for subsequent clinical experiments such as nucleic acid extraction or purification.
The collection method of Virus Transport Media:
1. Pharyngeal swab: Use two plastic rod swabs with polypropylene fiber heads to wipe both pharyngeal tonsils and back wall at the same time, immerse the swab heads in 3ml Virus Transport Media (also can use isotonic saline solution, tissue culture Discard the tail part of the tube with liquid or phosphate buffer solution), and screw the tube cap tightly.
2. Nasal swab: Gently insert a plastic rod swab with a polypropylene fiber head into the nasal palate in the nasal passage, stay for a while and then slowly turn it out. Take another plastic rod swab with polypropylene fiber head and collect the other nostril in the same way. The above two swabs were immersed in the same tube containing 30% of the sample liquid, the tail was discarded, and the tube cap was screwed tightly.
3. Nasopharyngeal aspiration or respiratory tract aspiration: Use a collector connected to a negative pressure pump to extract mucus from the nasopharynx or extract respiratory secretions from the trachea. Insert the head of the collector into the nasal cavity or trachea, turn on the negative pressure, rotate the head of the collector and slowly withdraw, collect the extracted mucus, and rinse the collector once with 3ml sampling solution (you can also use a pediatric catheter connected to a 50ml syringe Come up to replace the collector).
The cell preservation solution is a general-purpose cell cryopreservation solution, which can be used to freeze human and various animal cell lines; the complete cryopreservation solution formula is ready to use, convenient and fast; non-program cooling, -80℃ low temperature refrigerator freezing Store up to 5 years to ensure the safety of cryopreserved cells; cryopreservable plates (cell culture plates) can be used for cryopreservation of hybridoma cells. The cell preservation solution has a diluting effect and can be separated and embedded in mucus. The effective cells can be preserved, and provide sufficient cell numbers to ensure the accuracy of the test results. At the same time, the processed sample can be completely removed after the low-speed centrifugal filtration of the mucus in the sample, which is effective Prevent mucus from interfering with the sample test results. The cell preservation solution can be made into a uniform monolayer cell sheet after low-speed centrifugation, which meets the inspection requirements.
Apart from professionals, many people often confuse the cell preservation solution with Virus Transport Media, thinking that the two refer to the same preservation solution. In fact, the cell preservation solution is mainly used to freeze cells, and Virus Transport Media has There are two types of fire-extinguishing and non-extinguishing types. The fire-extinguishing type is mainly used for nucleic acid detection. For example, the more popular new coronavirus nucleic acid detection is the fire-extinguishing Virus Transport Media. The non-extinguishing type is mainly used for virus cultivation and reproduction. The biggest feature is that it can provide more accurate test results.
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