Among the pathogens (virus, bacteria, fungi, parasites, etc.) that cause the epidemic of human infectious diseases, virus is the most common one. Its most important feature is noncellular structure. The viral body is mainly composed of nucleic acid and protein. It contains only one nucleic acid. There is no enzyme system that can produce energy. It can only replicate in living cells. The virus is small and can be used for The unit of measurement is nanometer (nm), the maximum diameter is 300 nm (such as poxvirus), and the minimum diameter is 20 nm (such as foot-and-mouth disease virus). This characteristic of the virus makes it unable to survive without cells, and to study these viruses, we need to rely on the virus preservation solution to prolong the survival time of the virus.
At present, the virus preservation solution can be divided into inactivated type and non inactivated type
The left is non inactivated preservation solution, and the right is inactivated preservation solution
1. Inactivated novel coronavirus: it is a colorless transparent liquid, and is suitable for various kinds of viruses, including new coronavirus, influenza virus, hand foot mouth virus, rubella virus and so on. It uses high concentration guanidine salt to quickly inactivate and preserve the virus, so as to make the sample lose infectivity. The novel coronavirus RNA Extraction Kit novel coronavirus, M32/M96 nucleic acid extraction kit, and the new coronavirus PCR kit were successfully applied to detect the inactivated samples. The specificity of the assay was not affected. Applicable kit methods: fluorescent PCR, combined probe anchoring polymerization sequencing, isothermal amplification chip method, magnetic particle chemiluminescence method, colloidal gold method.
2. Non inactivated virus preservation solution: Hanks solution base, gentamicin, fungal antibiotic, BSA (V), and the other two were used as the main components, Cryoprotectants, biological buffers and amino acids, combined with a variety of antibiotics, have antibacterial and antifungal effects; bovine serum albumin (BSA), as a protein stabilizer, can form a protective membrane on the protein shell of the virus, making it not easy to decompose and ensure the integrity of the virus; the neutral environment constructed by Hanks buffer helps to increase the survival time of the virus and Infection stability.
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