Serum separation gel is a kind of hydrophobic organic compound, it is a viscous fluid, its structure contains a lot of hydrogen bonds, because of the association of hydrogen bonds to form a network structure. Under the action of centrifugal force, the network structure is destroyed and becomes a low viscosity fluid. When the centrifugal force disappears, the network structure is formed again and the fluid with high viscosity is recovered.
Serum separation gel is a material used to separate serum (plasma) and blood cells. Its main purpose is to form an isolation layer between blood cell components and serum, prevent material exchange between blood cells and serum, ensure the original characteristics of blood samples within a certain period of time, and make the detection results more accurate. In the field of clinical laboratory, no matter in clinical chemistry, serology, immunology, most of the samples need to be separated from the serum. It can also be used with heparin, EDTA and sodium citrate.
The key factors of separating gum are as follows
A. Specific gravity: 1.045-1.065g/cm3, between the specific gravity of serum (plasma) and blood cells. The PrP tube has two specific gravity of 1.055 and 1.078, which are used to separate platelet and serum components respectively;
B. Viscosity: between 100000-200000 Li poise (dynamic viscosity measured by rotary viscometer).
C. Thixotropy: when an object (such as a coating) is sheared, its consistency decreases. When it stops shearing, its consistency increases. When it is sheared, its consistency increases. When it stops shearing, its consistency decreases. That is, the property of changing at one touch. This is the key to the separation layer formed by centrifugal force.
D. Physiological inertia: the separation gel is in direct contact with the blood, and can not react with the blood, otherwise it will affect the blood composition, cause significant differences in test results, and lead to misdiagnosis. Blood cell is a kind of special delicate material, a little careless will most likely cause hemolysis, affect the accuracy of test results.
E. Radiation resistance: the blood collection vessel is required to be sterile, and gamma ray irradiation is generally used for sterilization. After gamma ray irradiation, the properties of the gel can not change significantly, including specific gravity, viscosity, thixotropy and physiological inertia. In general, gamma ray is produced by cobalt 60, and the radiation dose is generally in the range of 8-25kgy; the radiation dose is determined by the initial colony number of blood collection vessel.
G. Stability: on the one hand, it requires the stability of the separation adhesive after long-term storage, and it should be stable for at least two years, and the physical and chemical properties should not change significantly. In addition, there is no reaction between the separation gel and various additives in the blood collection vessel, which affects the effectiveness of the reagent and thus affects the blood detection.
Serum separation gel is still a glue with viscosity, and the viscosity has a certain relationship with temperature. When the temperature is too low and the weather is cold, the viscosity of serum gel increases, and the process of adding glue is difficult. In winter, the glue is heated. No problem under 80 ℃. Desheng has never stopped in the blood vessel additive. We want to use our products to tell all customers that your choice is right.
