Chemiluminescence immunoassay is currently the most advantageous immunoassay method. The core reagent is the chemiluminescence substrate. For example, the peroxidase substrate luminol and the alkaline phosphatase substrate AMPPD are widely used. There are acridine ester chemiluminescence reagents, which can directly generate chemiluminescence without enzyme catalysis.
Compared with chemiluminescence substrates such as luminol or alkaline phosphatase, acridine ester chemiluminescence reagents have obvious advantages. Its luminescence reaction does not require enzyme catalysis, and the luminescence detection step is simple and easy to automate; and In terms of sensitivity, acridine esters are many times higher than luminol and peroxyoxalate, and can detect a smaller amount and a wider range of detection indicators. Therefore, acridine ester chemiluminescent substrates are also more advanced immunoassay reagents.
What are the advantages of acridine esters compared with other chemiluminescent reagents
Acridine esters have a higher quantum yield. Compared with luminol, its chemiluminescence efficiency is five times or more than that of luminol; secondly, the properties of acridinium esters are more stable, and are affected by other substances in the reaction system. The impact is also smaller and storage is convenient. Thirdly, the chemiluminescence process of acridinium ester has a fast reaction speed, which can cause luminescence in the presence of hydrogen peroxide or other peroxides. The characteristics of the chemiluminescence reaction mechanism of acridinium esters show that the effect of light quenching is small, and the dissociation of the luminescent group and the luminous efficiency during the reaction will not be affected by the length of the connecting arm.
Acridinium esters and their derivatives can realize the chemiluminescence analysis of biological macromolecules such as antigens, antibodies and DNA, and are a kind of excellent chemiluminescence immunoassay labeling reagent. The main principle of action is to mark these biological macromolecules by directly or indirectly interacting with the amino, carboxyl or phosphoric acid groups on the biological macromolecules through the unique substituent groups on the molecular structure.
Among the many chemiluminescence reagents, the price of acridine esters is also the highest, but Desheng still provides luminol, isoluminol and other types of chemiluminescence reagents, which can meet the needs of different experimental detections. The luminescent reagent is not the most advanced, but the appropriate one.
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