Tris, Hepes, Mops and other buffers are almost all made up of buffer pairs composed of "weak acid and its conjugate base salt" or "weak base and its conjugate acid salt". You can add a certain amount of other buffers. A substance that slows down changes in PH. These two substances can be buffered toward acid or base at the same time because of the reaction. For example, a mixed solution of acetic acid and sodium acetate is a buffer solution. If hydrochloric acid is added, the pH will not drop too fast, because hydrochloric acid will react with sodium acetate to produce acetic acid. On the contrary, if sodium hydroxide is added, the pH will not increase too fast, because sodium hydroxide will react with acetic acid to form sodium acetate. In many chemical reactions, buffer solutions are used to keep the pH of the solution constant. Buffer solutions are of great significance to the production and evolution of life, because most organisms can only grow within a certain pH range.
There are many types of biological buffers, and here will introduce several commonly used biological buffers.
1. Tris buffer: Chinese name Tris(hydroxymethyl)aminomethane, CAS number 77-86-1, white crystalline powder, odorless and tasteless, good water solubility. It is a widely used buffer with a pKa of 8.1 at 25°C and a pH buffer range of 7.0-9.2. It is mostly used in chemical experiments, new coatings, cosmetics and other fields.
2. Hepes buffer: Chinese name 4-hydroxyethylpiperazine ethanesulfonic acid, CAS number 7365-45-9, white crystalline powder in appearance. It belongs to the "Good" buffer and is also one of the most suitable buffers for biological research. It is a zwitterionic organic buffer with a pH buffer range of 6.8-8.2. It is mostly used in cell culture, electrophoresis experiments, cosmetics and other fields.
3. Mops buffer: Chinese name 3-morpholine propane sulfonic acid, CAS number 1132-61-2, white powder in appearance. It is a zwitterionic buffer commonly used in biochemistry and molecular biology. It belongs to the "Good" buffer like Hepes. The pH buffer range of Mops is 6.5-7.9, and the pKa is 7.2 at 25°C. It is mostly used as physiologically related buffers, DNA/RNA extraction kits, PCR diagnostic kits, etc.
Nowadays, these buffers are often used in biochemical experiments, so the market demand for such buffers is very large. Therefore, buffer merchants on the market are mixed, making the procurement work very difficult. Recommend a manufacturer here: Hubei Xindesheng Material Co., Ltd. Desheng company has been producing chemical raw materials for decades. Desheng company has a professional team responsible for the development and production of biological buffers, and has imported equipment and raw materials for production and processing. The products produced by Desheng Company are of high quality, fast production efficiency, and sufficient stock in the warehouse. If you are interested in Desheng's products, you can enter the company's official website to contact customer service for details.
Contact Person: Miss. Ankiwang