What factors determine the result of chemiluminescence reagent reaction

Chemiluminescence is molecular electromagnetic radiation caused by light produced by a chemical reaction. Because of its high sensitivity, good specificity, wide application range, simple equipment and wide linear range, chemiluminescence reactions have received extensive attention in various fields of life sciences, detective detection, food safety, and drug analysis. A good chemiluminescence reaction is determined by the intensity and sensitivity of the reaction. The following is a brief analysis of the influencing factors.

Chemiluminescence labeling chemistry and labeling quantitative chemistry require high specific activity, and therefore require high detection sensitivity of the label material itself. The detection limit of conventional enzyme labels or fluorescent derivatives is about 1014 mol, which has been successfully applied to low-sensitivity measurement systems. The lower detection limit of luminol and acridine ester salts is about 10-18 mol of the available luminometer, so it matches or even exceeds the sensitivity of 1251.

Luminol and protein will greatly affect the quantum yield of the reaction. In particular, aminobutylethyl isoluminol can be combined with low molecular weight compounds without reducing the quantum yield. The compulsory method of first dissociation of the complex is to use the luminescence method. Quantum yield is an important indicator of the observation results when isoluminol is used to label hepatitis B antibodies.

When designing a detection scheme, there are many known chemiluminescence reactions to choose from. However, only a small number of known reactions have entered large-scale clinical diagnostic testing methods. There are several factors that influence the suitability of a particular chemiluminescence technique for automated immunoassays. First, the reaction should show sufficient chemiluminescence quantum yield.

All aspects of detection chemistry, including labeling methods, triggers, chemiluminescent reagents, are durable and reproducible. The method using enzyme labeling also faces another requirement, that is, the enzyme must be quite stable and easy to conjugate without reducing its catalytic activity. The ideal chemiluminescence reagent has an easy-to-measure signal through effective chemiluminescence reaction and predictable time course of luminescence.

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