In our nucleic acid examination, we will use our company's product: Virus Transport Media. In fact, the Virus Transport Media is also divided into multiple models, and there is also a twin brother: cell preservation solution.
Among them, the Virus Transport Media is roughly divided into two types: inactivated type and non-inactivated type. It can be understood from the literal meaning that there are differences in the preservation principles of the two types of Virus Transport Medias. Here is a brief introduction. The first non-inactivated type can protect the protein and nucleic acid of the virus, and the other is the inactivated type, which usually contains the lysis salt of the inactivated virus, which cleavages the protein to protect the nucleic acid. Among them, if the non-inactivated Virus Transport Media cannot be tested in time after sampling, it must be stored below 4 degrees Celsius and the preservation time should not exceed 48 hours. If it cannot be delivered to the test site within time, it needs to be Store in ultra-low temperature below minus 70 degrees Celsius. Otherwise, it will affect the virus test results, so you need to select the type of Virus Transport Media corresponding to the situation when selecting the Virus Transport Media.
Many people confuse the Virus Transport Media with the cell preservation solution. In fact, the cell preservation solution is a general-purpose cell cryopreservation solution that can be used to preserve animal and human cell lines. Cell preservation is a cytology experiment. Important technical means. In cell line establishment and line establishment, it is very important to freeze the original cells in time.
At present, cell preservation mostly uses glycerol or dimethyl sulfoxide protective agent, which can improve the permeability of the cell membrane to water, and then slow freezing can make the water in the cell seep out of the cell, reduce the formation of ice crystals in the cell, thereby reducing Cell damage caused by ice crystals. The cell preservation solution has a diluting effect, which can isolate a large number of effective cells embedded in the mucus, allowing more valuable cells to be preserved, providing a sufficient number of cells, and providing a guarantee for the accuracy of the test results.
Therefore, although the purpose of the Virus Transport Media and the cell preservation solution are similar, there are some differences in principle. Virus Transport Media is preserved by protecting nucleic acid, while cell preservation solution is preserved by improving cell capacity and protection. These two preservation solutions should be able to distinguish between them after the above understanding.
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