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What is the reason why the serum separation gel does not flip

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What is the reason why the serum separation gel does not flip
Latest company news about What is the reason why the serum separation gel does not flip

Serum separation gel is a special additive for blood collection that has viscosity and can form a gel like isolation layer under the action of centrifugal force, separating serum and blood clots, in order to better preserve serum and improve serum yield.

 

The method of flipping the serum separation gel is mainly during the centrifugation process. Usually, during the centrifugation process, the test tube is placed in a centrifuge, and the centrifugation speed and time are controlled to flip the serum separation gel under the action of centrifugal force.

 

When it comes to flipping, it has to be said that if the serum separation gel is not flipped, it may cause a series of problems. The serum and blood clots are not completely separated, and there may be impurities such as red blood cells and white blood cells in the serum, which affects the quality and purity of the serum.

 

Secondly, there may be residual coagulation factors such as fibrinogen in the serum, leading to incomplete blood coagulation and affecting the accuracy of experimental results. The most important thing is that the serum separation gel does not flip, which leads to separation failure and requires re collection of blood samples, increasing patient pain and medical costs.

 

Therefore, not flipping the serum separation gel is an abnormal phenomenon that needs to be dealt with in a timely manner to avoid adverse effects on the serum sample.

 

Since there are so many problems with not flipping the serum separation gel, let's talk about what causes the serum separation gel to not flip and how to effectively solve this problem when encountering it.

 

The failure or poor flipping of serum separation gel may be caused by the following reasons

 

1. Separation adhesive formula or process reasons. The different formulas and production processes of serum separation gel can lead to different performance indicators such as thixotropy, specific gravity, and stability, thereby affecting its flipping effect.

 

2. The separation adhesive ages and its thixotropic performance decreases. Thixotropy refers to the ability of serum separation gel to form a colloidal isolation layer under centrifugal force. If the thixotropy is not good, the serum separation gel cannot form a stable gel like isolation layer and cannot be flipped.

 

3. The initial centrifugal force of the separating adhesive is too large. The serum separation gel may be damaged and unable to form a stable gel like isolation layer.

 

4. If the centrifugal force is too small or the centrifugal time is too short, the serum separation gel may not be able to fully flip.

 

5. The minimum reversal centrifugal force of the separation gel is too high, which may also cause poor or inability to reverse the serum separation gel.

 

6. Temperature can affect the flipping of serum separation gel. When the temperature is too high, the viscosity of the serum separation gel decreases and the fluidity increases, making it difficult to form a stable gel like isolation layer, thereby affecting its flipping effect. When the temperature is too low, the viscosity of the serum separation gel increases, the fluidity decreases, and it can also cause poor or inability to flip.

 

7. Humidity has a certain impact on the turnover of serum separation gel. If the environmental humidity is too high, it may cause the serum separation gel to absorb water, thereby affecting its thixotropy and stability, and thus affecting its flipping effect.

 

Solution for non flipping or poor flipping of serum separation gel

 

1. Optimize the formula of separation adhesive and strictly control the synthesis process of separation adhesive.

 

2. Verify and confirm that the thixotropy of the separation adhesive meets the requirements within the validity period.

 

3. Improve the thixotropic performance of the separation adhesive and choose a suitable supplier.

 

4. Operate according to the manufacturer's recommended centrifugal requirements.

 

5. Pay attention to controlling the ambient temperature and the temperature during centrifugation.

 

In summary, the use of serum separation gel requires strict adherence to operating procedures to ensure safety and effectiveness. Therefore, professional consultation should be sought before use to ensure its correct use and safety. Details can be viewed on the official website of Desheng!

Pub Time : 2023-11-17 11:08:53 >> News list
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