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What are the types of Virus Transport Medias?

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What are the types of Virus Transport Medias?

Since the outbreak of the new coronavirus, the Virus Transport Media has played an important role. First, the nasal and pharyngeal swabs were used to sample, and the samples were put into the sampling tube added with the Virus Transport Media for sample protection, which improved the accuracy of nucleic acid detection. Virus Transport Media is a solution medium used for virus sampling, transportation, storage and detection. If it is added into the virus sampling tube, what kinds of Virus Transport Media are added?

 

There are two kinds of Virus Transport Medias, inactivated and non inactivated. There are many kinds of Virus Transport Medias on the market. The following is a brief introduction of several principles for the selection of Virus Transport Medias.

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Non inactivated preservation solution:

Based on Hank's, the non inactivated virus does not contain lysate, and is supplemented with nutrients needed by virus, such as BSA (bovine serum albumin) amino acids, vitamins, etc. it can maintain the activity of virus in a wide temperature range and keep the original sample to the greatest extent. It can be used for virus nucleic acid extraction and detection, virus culture and isolation.

 

Characteristics of non inactivated preservation solution:

1. The virus can remain active and can be used for detection or virus isolation;

2. There was no inhibition on subsequent nucleic acid amplification, which could be matched with direct amplification reagent;

3. After the swab is added, it needs to be refrigerated to detect the main components as soon as possible.

 

Inactivated preservation solution:

Inactivation preservation solution can fully mix the collected samples with virus lysate and virus nucleic acid preservation solution to inactivate the virus in the samples, and effectively ensure the integrity of virus nucleic acid in the samples. The collected samples can be transported under normal temperature and preserved for a long time. The preserved virus RNA samples can be widely used in gene detection, enzyme-linked immunosorbent assay (ELISA), PCR detection and so on. The inactivation preservation solution can be divided into guanidine containing solution and non guanidine containing solution.

 

1. Guanidine salt

(1) The virus can be split and inactivated instantaneously to reduce the risk of sampling;

(2) The viral nucleic acid could be stored at room temperature for 2 weeks;

(3) It can match most nucleic acid extraction reagents.

 

2. Non guaiac salt

(1) The virus can be inactivated instantaneously to reduce the risk of sampling, and the virus may recover its infectivity after dilution;

(2) The viral nucleic acid could be stored at room temperature for 2 weeks;

(3) It can match most nucleic acid extraction reagents.

 

3. Direct expansion preservation solution

(1) The virus can be inactivated instantaneously to reduce the risk of sampling, and the virus may recover its infectivity after dilution;

(2) The viral nucleic acid can be stored in cold storage for 24-48 hours;

(3) It can be directly added into nucleic acid amplification system as template;

(4) Most of the nucleic acid extraction reagents and releasing agents were matched.

 

I believe that after reading the above explanation, we will have more basis for choosing the correct and applicable preservation solution. Desheng is a manufacturer of Virus Transport Media, which can provide inactivated and non inactivated preservation solutions. We can accept customized samples from customers, and also provide disposable virus sampling tube and nasopharyngeal swab, etc. to provide customers with one-stop service. If you need, you can click the official website for consultation or contact directly Our professional sales staff.

Pub Time : 2021-02-27 20:22:55 >> News list
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