The EDTA anticoagulant blood routine test is the most basic test for blood testing, but whether it is better to use venous blood or peripheral blood for the test, there are different opinions in the industry. Blood routine involves the detection of multiple indicators, and it is necessary to compare the data of each indicator measured by two blood sampling methods.
The popularity and application of blood analyzers have greatly improved the level and efficiency of blood testing, laboratory science continues to develop, and the test results are more accurate. After comparing the data reproducibility and corresponding data of the two blood samples, it is found that the data of venous blood collection has good reproducibility, while the reproducibility of peripheral blood collection is relatively poor, and the data obtained by the two methods are not the same.
Repeatability test results: There is no significant difference in the repeatability of the white blood cell (WBC), red blood cell (RBC), and hemoglobin (HGB) test results of venous blood sampling (P>0.05), while the difference in the repeatability of peripheral blood sampling is significant (P<0.01) .
Comparison of blood routine test results: the white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), platelet (PLT) test results were significantly different (P<0.01).
After investigation and analysis, the reasons for the difference between the results of venous blood sampling and peripheral blood sampling are summarized:
1. The measurement of peripheral blood RBC, HGB, PLT is lower than venous blood, and WBC is higher than venous blood. It may be that the temperature of peripheral blood is lower than that of venous blood when collecting finger blood. The local reaction of piercing finger is obvious, causing WBC to rise. high.
2. When the peripheral blood of the finger is taken, it is likely to be mixed into the tissue fluid, causing platelet aggregation, resulting in a false reduction of platelets.
3. The various components of venous blood are not easily affected by environmental changes, and can better reflect the blood condition of the whole body, so the repeatability is higher than that of peripheral blood sampling. The peripheral blood includes arteriovenous blood, capillary blood, interstitial fluid and intracellular fluid.
4. In order to obtain sufficient peripheral blood, it is often necessary to repeatedly squeeze, forming a strong stimulus, causing tension in the blood sampled, and accelerating platelet aggregation and affecting the test results.
In summary, when EDTA anticoagulant tube is used for routine blood testing, the results of venous blood sampling are better than peripheral blood sampling in all aspects. As a blood test reagent manufacturer, Desheng advocates that everyone try to use venous blood for blood analysis.
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