TOOS is also called EHSPT (N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt), is a highly water-soluble sodium salt of aniline, the sensitivity of the Trinder reaction It is much higher than traditional phenol, aniline, ADA and other color-developing substrates. Clinical biochemical testing is often used in liver function kits, blood glucose metabolism kits, etc.
In the presence of hydrogen peroxide and peroxidase POD, Trinder's reagent forms a very stable orange or red quinone with 4-aminoantipyrine (4-AAP). The molar absorbance of TOOS and MBTH coupling dye is 1.5-2 times higher than that of 4-AA coupling dye; however, 4-AAP solution is more stable than MBTH solution, and Trinder’s reagent is more used with 4-AAP.
Color substrate reagent TOOS
When tested with a biochemical kit, the measured indicators such as uric acid, glucose, glycated albumin, and 1,5-anhydroglucitol are equal to the enzymatic oxidation of its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the concentration of the test substance Therefore, the amount of the analyte index can be determined by the color development of the oxidative coupling reaction. Of course, enzyme activity indicators can also be measured, such as adenosine dehydrogenase detection kit and 5'-nucleotidase detection in liver function series. It is through the reaction of the enzyme to be detected and its corresponding catalytic substance to generate hydrogen peroxide, and then through the coloring substrate TOOS coupling 4-AAP to react with the generated hydrogen peroxide, the final rate of production of the coloring product corresponds to the measured Enzyme activity, so as to achieve the purpose of measuring indicators.
The TOOS color development substrate developed by Desheng Technology has the characteristics of high purity, high water solubility, and low interference impurity ions. It can be quickly configured during use, which is very convenient; and the company also produces Tris buffer used with it for biochemical detection. Wait, the quality and service are well received!
