TOOS is also called EHSPT (N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt), which is a highly water-soluble sodium salt of aniline. It is sensitive when performing Trinder reaction. It is much higher than traditional phenol, aniline, ADA and other color-developing substrates. Clinical biochemical testing is often used in liver function kits, blood glucose metabolism kits, etc.
In the presence of hydrogen peroxide and peroxidase POD, Trinder's reagent forms a very stable orange or red quinone with 4-aminoantipyrine (4-AAP). The molar absorbance of TOOS and MBTH coupling dye is 1.5-2 times higher than that of 4-AA coupling dye; however, 4-AAP solution is more stable than MBTH solution, so Trinder ’s reagent is more used with 4-AAP.
When tested with a biochemical kit, the measured indicators such as uric acid, glucose, glycated albumin, and 1,5-anhydroglucitol are equal to the enzymatic oxidation of its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the concentration of the substance to be tested Therefore, the amount of the analyte index can be determined by the color development of the oxidative coupling reaction. Certainly, the indicators of enzyme activity can also be measured, such as the adenosine dehydrogenase detection kit and 5'-nucleotidase detection in the liver function series, which is the reaction of the enzyme to be detected and its corresponding catalytic substance to generate peroxide Hydrogen is then reacted with the generated hydrogen peroxide through the coloring substrate TOOS coupling 4-AAP, and the final coloring product production rate corresponds to the activity of the tested enzyme, thereby achieving the purpose of measuring the index.
The TOOS color development substrate developed by Desheng Technology has the characteristics of high purity, high water solubility, and low interference ion. It is very convenient to use when it is used, and the company also produces Tris buffer used with it for biochemical detection. Wait, the quality and service are well received!
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