Toos, n-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3-methylaniline sodium salt, is a widely used color reagent, which is most commonly used in the new Trinder's reagent. Toos has been used in many clinical biochemical test items. This article will introduce the specific test items for which the chromogenic reagent toos can be used.
Through Trinder reaction, toos is widely used in diagnostic detection and biochemical tests. Trinder reaction is also known as "coupling end point colorimetry". The principle is that hydrogen peroxide (H2O2) produced by enzyme reaction can generate red quinoline imine compound in the presence of 4-aminoantipyrine (4-AAP) and peroxidase (POD). The specific detection application of toos mainly includes blood glucose detection And liver function test, the following is a brief introduction to the detection principle of these two methods.
TOOS, the substrate of Desheng chromogen
1. Blood glucose detection project: toos is used to prepare glucose detection reagent and glycosylated albumin detection reagent in blood glucose metabolism project. According to the description of patent cn105572065a, glucose oxidase is used to catalyze the oxidation of glucose to hydrogen peroxide, and platinum bismuth nano mimetic peroxidase is used to catalyze the oxidation of 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) and sodium salt of n-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3-methylaniline (toos) by hydrogen peroxide. The color of the color developing product solution is purple, and with the concentration of glucose increasing The maximum absorption wavelength of the color system is 590 nm, and the content of glucose can be obtained from the absorbance at 590 nm.
2. Routine examination items of liver function: adenosine deaminase (ADA) activity is a sensitive index reflecting liver injury, and is one of the routine examination items of liver function. The detection reagent of adenosine deaminase composed of toos, bovine serum albumin, disodium ethylenediamine tetraacetate, etc., has the advantages of good color effect, rapid response, stability and high precision.
In addition, toos is also used in the diagnostic reagents for triglyceride and other blood lipid detection items and cholesterol detection items, which has important value in clinical diagnosis. The toos chromogenic reagent produced by Desheng is not only of high purity, but also of high quality, High sensitivity, and in the absorption wavelength, color reaction intensity and fading have good performance, welcome the majority of scientific research workers and dealers colleagues to consult and purchase!
Contact Person: Miss. Ankiwang