After the virus sample is collected, usually the sample collection place can not carry out PCR detection in time, so the collected virus swab sample needs to be transported, and the virus itself will soon be cleaved in vitro to affect subsequent detection, so it is stored and transported. At this time, it is necessary to add a virus transportation media, which is currently mainly divided into inactivated and activated types.
For different detection purposes, different virus transportation media need to be used, and the two widely used transportation media have their own characteristics. In order to meet different testing requirements and different virus testing laboratory conditions, it is necessary to apply different virus transportation media.
Inactivated virus transportation media:
It is mainly a modified virus lysate transportation media of nucleic acid extraction lysate. The high concentration of guanidine salt in the sample can effectively inactivate the virus, which can effectively prevent the operator's secondary infection, but also contains the Rnase inhibitor, which can protect the viral nucleic acid from being degraded, so that it can be detected by NT-PCR. Moreover, it can be stored for a relatively long time at normal temperature, saving the cost of virus sample storage and transportation.
Activated virus transportation media:
It is mainly based on the modified virus maintenance liquid type transportation media. It can maintain the activity of the virus in vitro and the integrity of antigens and nucleic acids. It contains Hanks' liquid foundation, gentamicin, fungal antibiotics, BSA (V), cryoprotectants, biological buffers, and amino acids. Decomposition, to maintain the originality of the virus sample to a large extent. In addition to nucleic acid extraction and detection, this transportation meida can also be used for virus cultivation, isolation, and antigen detection. However, it must be kept at a strictly low temperature for long-term storage after sampling.
It can be seen that according to different situations, different types of transportation media have their own advantages and disadvantages. However, it should be noted that no matter whether it is an inactivated type or a activated type, the virus transportation tube before sampling must be strictly inactivated and sterilized to ensure that there are no other microorganisms in the tube, resulting in erroneous detection of virus decomposition or other effects after sampling.
After the swab is sampled, if you use a collection tool or transportation media of insufficient quality, it will affect the subsequent test results and even cause false positives for misdiagnosis. Therefore, it is recommended to use a high-quality virus transportation media, such as a professional biochemical reagent manufacturer like Desheng Technology In this aspect, it has rich R&D and management experience and is worthy of market trust!
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