N-Tris(Hydroxymethyl)Methyl-3-Aminopropanesulfonic Acid (TAPS Buffer) is a zwitterionic buffer widely used in the fields of biochemistry and molecular biology, CAS number: 29915-38-6, and the pH buffer range of TAPS is 7.7 -9.1, soluble in water (25g/50ml).
In clinical diagnosis, TAPS Buffer is used as a biological buffer in biochemical diagnostic kits. So what factors can affect the yield and purity of TAPS Buffer?
The reaction time, different alcohol solvents and the recycling of reaction solvents have a certain degree of influence on various types of biological buffers, and the same is true for TAPS Buffer. The experimental results show that the reaction time is controlled at 6 hours, and the yield is the highest, reaching 70.3%. Continue to extend the reaction time, and the yield will decrease slightly; for the investigated ethanol, n-propanol, isopropanol and n-butanol reaction solvents, n-butanol As the reaction solvent, the yield and purity of TAPS are the highest, 70.3% and 94.36%, respectively; in the reaction process, the recycling of the reaction solvent is beneficial to improve the yield and purity of TAPS Buffer.
TAPS Buffer reagent
TAPS Buffer is a commonly used buffer system in DNA screening systems, and also as a buffer component of RNA samples. It is suitable for electron transport and phosphorylation studies of chloroplast thin-layer preparations, and protects oxygenated hemoglobin from oxidation during freeze-drying. Methemoglobin can also be used as a background electrolyte for protein microanalysis in capillary zone electrophoresis.
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