TOOS, also known as EHSPT (N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt), is a highly water-soluble aniline sodium salt. When Trinder reaction is carried out, the sensitivity is much higher than that of traditional chromogenic substrates such as phenol, aniline and ADA.Clinical biochemical tests are often used in liver function kits, blood glucose metabolism kits, etc.
In the presence of hydrogen peroxide and peroxidase POD, Trinder's reagent forms very stable orange or red quinones with 4-aminoantipyrine (4-AAP).The molar absorbance of TOOS and MBTH-coupled dyes is 1.5-2 times higher than that of 4-AA-coupled dyes; however, 4-AAP solution is more stable than MBTH solution, so Trinder's reagent is more used with 4-AAP.
TOOS chromogenic substrate reagent
The measured indicators such as uric acid, glucose, glycated albumin and 1,5-dehydroglucitol are equal to the hydrogen peroxide produced by the enzymatic oxidation of their oxidase, and the concentration of hydrogen peroxide corresponds to the concentration of the substance to be measured. Therefore, the amount of the substance to be measured can be determined by the color development of the oxidative coupling reaction.Of course, the indicators of enzyme activities can also be measured, such as adenosine dehydrogenase detection kit in the liver function series, 5'-nucleotidase detection, that is, hydrogen peroxide is generated by the reaction of the enzyme to be detected with its corresponding catalytic substance, and then 4-AAP is coupled with the generated hydrogen peroxide by the chromogenic substrate TOOS, the final production rate of the chromogenic product corresponds to the activity of the tested enzyme.So as to achieve the purpose of measuring indicators.
The TOOS chromogenic substrate developed by DESHENG Technology has the characteristics of high purity, high water solubility and less interference impurity ions. It is convenient to use it in combination with ready-to-use. In addition, the company also produces Tris buffer which is used in combination with biochemical detection. The quality and service are widely praised!